Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1975 Feb 1;141(2):335–345. doi: 10.1084/jem.141.2.335

The presence of IgA on the surface of rat thoractic duct lymphocytes which contain internal IgA

PMCID: PMC2190527  PMID: 1089743

Abstract

The presence of lymphocytes with internal IgA among cells from rat thoracic duct lymph wdy. The number of cells detected was greater in animals kept in a convential animal house compared with those maintained under specific pathogen-free conditions. Thoracic duct lymph from B rats and adult thymectomized rats also contained cell with internal IgA. The surface Ig of the IgA-containing cells was studied using a double-labeling technique with (126I) anti-Ig to detect surface Ig, and fluorescein-conjugated anti-IgA in large amounts, but very little IgM and no surface IgG2. The surface IgA was not acquired passively.

Full Text

The Full Text of this article is available as a PDF (628.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bazin H., Beckers A., Querinjean P. Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur J Immunol. 1974 Jan;4(1):44–48. doi: 10.1002/eji.1830040112. [DOI] [PubMed] [Google Scholar]
  2. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  3. GOWANS J. L. The recirculation of lymphocytes from blood to lymph in the rat. J Physiol. 1959 Apr 23;146(1):54–69. doi: 10.1113/jphysiol.1959.sp006177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gowans J. L. Differentiation of the cells which synthesize the immunoglobulins. Ann Immunol (Paris) 1974 Jan;125C(1-2):201–211. [PubMed] [Google Scholar]
  5. Griscelli C., Vassalli P., McCluskey R. T. The distribution of large dividing lymph node cells in syngeneic recipient rats after intravenous injection. J Exp Med. 1969 Dec 1;130(6):1427–1451. doi: 10.1084/jem.130.6.1427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hall J. G., Parry D. M., Smith M. E. The distribution and differentiation of lymph-borne immunoblasts after intravenous injection into syngeneic recipients. Cell Tissue Kinet. 1972 May;5(3):269–281. doi: 10.1111/j.1365-2184.1972.tb00365.x. [DOI] [PubMed] [Google Scholar]
  7. Howard J. C. The life-span and recirculation of marrow-derived small lymphocytes from the rat thoracic duct. J Exp Med. 1972 Feb 1;135(2):185–199. doi: 10.1084/jem.135.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hunt S. V., Williams A. F. The origin of cell surface immunoglobulin of marrow-derived and thymus-derived lymphocytes of the rat. J Exp Med. 1974 Mar 1;139(3):479–496. doi: 10.1084/jem.139.3.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jensenius J. C., Williams A. F. The binding of anti-immunoglobulin antibodies to rat thymocytes and thoracic duct lymphocytes. Eur J Immunol. 1974 Feb;4(2):91–97. doi: 10.1002/eji.1830040207. [DOI] [PubMed] [Google Scholar]
  10. Jensenius J. C., Williams A. F. Total immunoglobulin of rat thymocytes and thoracic duct lymphocytes. Eur J Immunol. 1974 Feb;4(2):98–105. doi: 10.1002/eji.1830040208. [DOI] [PubMed] [Google Scholar]
  11. Listing F. Stardardized nomenclature for inbred strains of rats. Transplantation. 1973 Sep;16(3):221–245. [PubMed] [Google Scholar]
  12. MANN J. D., HIGGINS G. M. Lymphocytes in thoracic duct intestinal and hepatic lymph. Blood. 1950 Feb;5(2):177–190. [PubMed] [Google Scholar]
  13. Mandel M. A., Asofsky R. Studies of thoracic duct lymphocytes of mice. I. Immunoglobulin synthesis in vitro. J Immunol. 1968 Feb;100(2):363–370. [PubMed] [Google Scholar]
  14. Moore A. R., Hall J. G. Evidence for a primary association between immunoblasts and small gut. Nature. 1972 Sep 15;239(5368):161–162. doi: 10.1038/239161a0. [DOI] [PubMed] [Google Scholar]
  15. Ogra P. L., Karzon D. T. Distribution of poliovirus antibody in serum, nasopharynx and alimentary tract following segmental immunization of lower alimentary tract with poliovaccine. J Immunol. 1969 Jun;102(6):1423–1430. [PubMed] [Google Scholar]
  16. Poger M. E., Lamm M. E. Localization of free and bound secretory component in human intestinal epithelial cells. A model for the assembly of secretory IgA. J Exp Med. 1974 Mar 1;139(3):629–642. doi: 10.1084/jem.139.3.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. RIGGS J. L., SEIWALD R. J., BURCKHALTER J. H., DOWNS C. M., METCALF T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum. Am J Pathol. 1958 Nov-Dec;34(6):1081–1097. [PMC free article] [PubMed] [Google Scholar]
  18. Uhr J. W., Vitetta E. S. Cell surface immunoglobulin. 8. Synthesis, secretion, and cell surface expression of immunoglobulin in murine thoracic duct lymphocytes. J Exp Med. 1974 Apr 1;139(4):1013–1018. doi: 10.1084/jem.139.4.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vaerman J. P., André C., Bazin H., Heremans J. F. Mesenteric lymph as a major source of serum IgA in guinea pigs and rats. Eur J Immunol. 1973 Sep;3(9):580–584. doi: 10.1002/eji.1830030911. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES