Due to a printing error, Fig. 5 reproduced poorly in the Journal and information was lost. The corrected Fig. 5 appears below:
Figure 5.
ugo1Δ dnm1Δ cells contain mitochondrial tubules and maintain mtDNA. Wild-type (FY833, WT), ugo1Δ (YHS72), dnm1Δ (YHS83), and ugo1Δ dnm1Δ (YHS85) cells expressing OM45-GFP (pKC2) were grown to log phase in SRaf medium, stained with 1 μg/ml DAPI, and viewed by DIC and fluorescence (OM45-GFP) microscopy. (B) Wild-type, ugo1Δ, dnm1Δ, and ugo1Δ dnm1Δ cells were grown to log phase in YEPD medium. Cells were collected and resuspended in YEPD medium to an OD600 of 2. Cells were then diluted in 10-fold increments, and 10 μl of each dilution was spotted onto YEPD and YEPGE media and incubated at 30°C for 2 and 6 d, respectively. N, nuclear DNA staining. Bar, 3 μm.