Abstract
We have previously described the isolation of a cDNA clone, designated NKG2, that was expressed in all natural killer (NK) cells tested but not in T or B cells. In the present communication, the original isolate, when used to probe a cDNA library prepared from a CD3- NK cell clone, was found to crosshybridize with a family of transcripts that fell into four distinct groups designated NKG2-A, -B, -C, and -D. Full- length cDNA sequences were determined for each group, and the DNA and inferred peptide sequences were analyzed. All four transcripts encode type II membrane proteins of 215-233 amino acids. NKG2-A and -B peptides appear to be alternative splicing products of a single gene. NKG2-C is highly homologous with group A, having 94% homology in the external (COOH-terminal) domain and 56% homology throughout the internal and transmembrane regions. NKG2-D is distantly but significantly related (21% amino acid homology) to the first three groups. Therefore, NKG2-A, -C, and -D appear to be encoded by distinct genes within a family of NK cell-specific genes. Peptide sequence homology searches demonstrate that the NKG2 peptides are members of a supergene family that includes several other type II membrane proteins. This family is characterized by the presence of a C-type animal lectin domain, and several of its members have demonstrated transmembrane signaling capability.
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Selected References
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- Chambers W. H., Vujanovic N. L., DeLeo A. B., Olszowy M. W., Herberman R. B., Hiserodt J. C. Monoclonal antibody to a triggering structure expressed on rat natural killer cells and adherent lymphokine-activated killer cells. J Exp Med. 1989 Apr 1;169(4):1373–1389. doi: 10.1084/jem.169.4.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drickamer K. Two distinct classes of carbohydrate-recognition domains in animal lectins. J Biol Chem. 1988 Jul 15;263(20):9557–9560. [PubMed] [Google Scholar]
- Giorda R., Rudert W. A., Vavassori C., Chambers W. H., Hiserodt J. C., Trucco M. NKR-P1, a signal transduction molecule on natural killer cells. Science. 1990 Sep 14;249(4974):1298–1300. doi: 10.1126/science.2399464. [DOI] [PubMed] [Google Scholar]
- Houchins J. P., Yabe T., McSherry C., Miyokawa N., Bach F. H. Isolation and characterization of NK cell or NK/T cell-specific cDNA clones. J Mol Cell Immunol. 1990;4(6):295–306. [PubMed] [Google Scholar]
- Singer S. J., Maher P. A., Yaffe M. P. On the transfer of integral proteins into membranes. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1960–1964. doi: 10.1073/pnas.84.7.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spiess M., Lodish H. F. Sequence of a second human asialoglycoprotein receptor: conservation of two receptor genes during evolution. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6465–6469. doi: 10.1073/pnas.82.19.6465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trinchieri G. Biology of natural killer cells. Adv Immunol. 1989;47:187–376. doi: 10.1016/S0065-2776(08)60664-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yokoyama W. M., Jacobs L. B., Kanagawa O., Shevach E. M., Cohen D. I. A murine T lymphocyte antigen belongs to a supergene family of type II integral membrane proteins. J Immunol. 1989 Aug 15;143(4):1379–1386. [PubMed] [Google Scholar]
- Yokoyama W. M., Kehn P. J., Cohen D. I., Shevach E. M. Chromosomal location of the Ly-49 (A1, YE1/48) multigene family. Genetic association with the NK 1.1 antigen. J Immunol. 1990 Oct 1;145(7):2353–2358. [PubMed] [Google Scholar]