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. 1993 Apr 1;177(4):1193–1198. doi: 10.1084/jem.177.4.1193

Lethal exacerbation of Pneumocystis carinii pneumonia in severe combined immunodeficiency mice after infection by pneumonia virus of mice

PMCID: PMC2190992  PMID: 8459214

Abstract

Mice homozygous for the mutant allele scid (severe combined immunodeficiency) have been described as excellent models for Pneumocystis carinii (Pc) pneumonia (PCP), a major health problem in patients with acquired immune deficiency syndrome (AIDS) and other immunodeficiency states. Other microorganisms have been shown to infect AIDS patients simultaneously with Pc, but whether one opportunist is able to directly influence the pathogenicity of another has not been determined previously. We have deliberately coinfected scid mice (with extent Pc infection) with a variety of primarily pneumotropic viruses and bacteria and have identified pneumonia virus of mice as causing a dramatic increase in the density of Pc organisms and the morbidity due to PCP in immunodeficient scid mice. This finding has clinical significance in the management of PCP, in that the identification and treatment of coinfecting pneumotropic pathogens may be as important as treatment targeted at Pc. A search for other synergistic (or antagonistic) microorganisms and determination of their mechanism(s) of action in altering the progression of PCP is indicated.

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Selected References

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  1. Barthold S. W., Smith A. L., Lord P. F., Bhatt P. N., Jacoby R. O., Main A. J. Epizootic coronaviral typhlocolitis in suckling mice. Lab Anim Sci. 1982 Aug;32(4):376–383. [PubMed] [Google Scholar]
  2. Brownstein D. G., Smith A. L., Johnson E. A. Sendai virus infection in genetically resistant and susceptible mice. Am J Pathol. 1981 Nov;105(2):156–163. [PMC free article] [PubMed] [Google Scholar]
  3. Cash P., Wunner W. H., Pringle C. R. A comparison of the polypeptides of human and bovine respiratory syncytial viruses and murine pneumonia virus. Virology. 1977 Oct 15;82(2):369–379. doi: 10.1016/0042-6822(77)90012-5. [DOI] [PubMed] [Google Scholar]
  4. Chambers P., Pringle C. R., Easton A. J. Genes 1 and 2 of pneumonia virus of mice encode proteins which have little homology with the 1C and 1B proteins of human respiratory syncytial virus. J Gen Virol. 1991 Oct;72(Pt 10):2545–2549. doi: 10.1099/0022-1317-72-10-2545. [DOI] [PubMed] [Google Scholar]
  5. Edman J. C., Kovacs J. A., Masur H., Santi D. V., Elwood H. J., Sogin M. L. Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the fungi. Nature. 1988 Aug 11;334(6182):519–522. doi: 10.1038/334519a0. [DOI] [PubMed] [Google Scholar]
  6. Kovacs J. A., Masur H. Pneumocystis carinii pneumonia: therapy and prophylaxis. J Infect Dis. 1988 Jul;158(1):254–259. doi: 10.1093/infdis/158.1.254. [DOI] [PubMed] [Google Scholar]
  7. Krishnan V. L., Meager A., Mitchell D. M., Pinching A. J. Alveolar macrophages in AIDS patients: increased spontaneous tumour necrosis factor-alpha production in Pneumocystis carinii pneumonia. Clin Exp Immunol. 1990 May;80(2):156–160. doi: 10.1111/j.1365-2249.1990.tb05225.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ling R., Pringle C. R. Polypeptides of pneumonia virus of mice. I. Immunological cross-reactions and post-translational modifications. J Gen Virol. 1989 Jun;70(Pt 6):1427–1440. doi: 10.1099/0022-1317-70-6-1427. [DOI] [PubMed] [Google Scholar]
  9. Pesanti E. L. Effects of bacterial pneumonitis on development of pneumocystosis in rats. Am Rev Respir Dis. 1982 Jun;125(6):723–726. doi: 10.1164/arrd.1982.125.6.723. [DOI] [PubMed] [Google Scholar]
  10. Prince G. A., Horswood R. L., Berndt J., Suffin S. C., Chanock R. M. Respiratory syncytial virus infection in inbred mice. Infect Immun. 1979 Nov;26(2):764–766. doi: 10.1128/iai.26.2.764-766.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reichert C. M., O'Leary T. J., Levens D. L., Simrell C. R., Macher A. M. Autopsy pathology in the acquired immune deficiency syndrome. Am J Pathol. 1983 Sep;112(3):357–382. [PMC free article] [PubMed] [Google Scholar]
  12. Richter C. B., Thigpen J. E., Richter C. S., Mackenzie J. M., Jr Fatal pneumonia with terminal emaciation in nude mice caused by pneumonia virus of mice. Lab Anim Sci. 1988 Jun;38(3):255–261. [PubMed] [Google Scholar]
  13. Roths J. B., Marshall J. D., Allen R. D., Carlson G. A., Sidman C. L. Spontaneous Pneumocystis carinii pneumonia in immunodeficient mutant scid mice. Natural history and pathobiology. Am J Pathol. 1990 May;136(5):1173–1186. [PMC free article] [PubMed] [Google Scholar]
  14. Roths J. B., Sidman C. L. Both immunity and hyperresponsiveness to Pneumocystis carinii result from transfer of CD4+ but not CD8+ T cells into severe combined immunodeficiency mice. J Clin Invest. 1992 Aug;90(2):673–678. doi: 10.1172/JCI115910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shann F., Walters S., Pifer L. L., Graham D. M., Jack I., Uren E., Birch D., Stallman N. D. Pneumonia associated with infection with pneumocystis, respiratory syncytial virus, chlamydia, mycoplasma, and cytomegalovirus in children in Papua New Guinea. Br Med J (Clin Res Ed) 1986 Feb 1;292(6516):314–317. doi: 10.1136/bmj.292.6516.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith A. L. An immunofluorescence test for detection of serum antibody to rodent coronaviruses. Lab Anim Sci. 1983 Apr;33(2):157–160. [PubMed] [Google Scholar]
  17. Smith A. L., Carrano V. A., Brownstein D. G. Response of weanling random-bred mice to infection with pneumonia virus of mice (PVM). Lab Anim Sci. 1984 Feb;34(1):35–37. [PubMed] [Google Scholar]
  18. Stover D. E., White D. A., Romano P. A., Gellene R. A., Robeson W. A. Spectrum of pulmonary diseases associated with the acquired immune deficiency syndrome. Am J Med. 1985 Mar;78(3):429–437. doi: 10.1016/0002-9343(85)90334-1. [DOI] [PubMed] [Google Scholar]
  19. Stringer S. L., Stringer J. R., Blase M. A., Walzer P. D., Cushion M. T. Pneumocystis carinii: sequence from ribosomal RNA implies a close relationship with fungi. Exp Parasitol. 1989 May;68(4):450–461. doi: 10.1016/0014-4894(89)90130-6. [DOI] [PubMed] [Google Scholar]
  20. Weir E. C., Brownstein D. G., Smith A. L., Johnson E. A. Respiratory disease and wasting in athymic mice infected with pneumonia virus of mice. Lab Anim Sci. 1988 Apr;38(2):133–137. [PubMed] [Google Scholar]

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