Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Jul;163(1):349–354. doi: 10.1128/jb.163.1.349-354.1985

Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA.

Y Hayakawa, T Murotsu, K Matsubara
PMCID: PMC219120  PMID: 3891736

Abstract

A mini-F-coded protein, named F2 protein, binds specifically to mini-F DNA. This protein has a molecular weight of 37,000 and is coded by the A2 segment of the mini-F genome (47.3 to 49.4 kilobases on the F coordinate map). The binding site is located also in the A2 segment of mini-F. This binding site is lost by spontaneous deletion when the A2 segment alone, but not A2 together with its neighboring segment, is cloned in a multicopy plasmid pBR322. These data are discussed in connection with incompatibility and plasmid stability.

Full text

PDF
349

Images in this article

351Image
on p.351

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austin S., Wierzbicki A. Two mini-F-encoded proteins are essential for equipartition. Plasmid. 1983 Jul;10(1):73–81. doi: 10.1016/0147-619x(83)90059-8. [DOI] [PubMed] [Google Scholar]
  2. Bex F., Karoui H., Rokeach L., Drèze P., Garcia L., Couturier M. Mini-F encoded proteins: identification of a new 10.5 kilodalton species. EMBO J. 1983;2(11):1853–1861. doi: 10.1002/j.1460-2075.1983.tb01671.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eichenlaub R., Figurski D., Helinski D. R. Bidirection replication from a unique origin in a mini-F plasmid. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138–1141. doi: 10.1073/pnas.74.3.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gardner R. C., Malcolm L., Bergquist P. L., Lane H. E. IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group. Mol Gen Genet. 1982;188(2):345–352. doi: 10.1007/BF00332699. [DOI] [PubMed] [Google Scholar]
  5. Kline B. C., Lane D. A proposed system for nomenclature for incompatibility genes of the Escherichia coli sex factor, plasmid F. Plasmid. 1980 Sep;4(2):231–232. doi: 10.1016/0147-619x(80)90013-x. [DOI] [PubMed] [Google Scholar]
  6. Komai N., Nishizawa T., Hayakawa Y., Murotsu T., Matsubara K. Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments. Mol Gen Genet. 1982;186(2):193–203. doi: 10.1007/BF00331850. [DOI] [PubMed] [Google Scholar]
  7. Kwoh D., Zipser D. Specific binding of mu repressor to DNA. Nature. 1979 Feb 8;277(5696):489–491. doi: 10.1038/277489a0. [DOI] [PubMed] [Google Scholar]
  8. Lane H. E. Replication and incompatibility of F and plasmids in the IncFI Group. Plasmid. 1981 Jan;5(1):100–126. doi: 10.1016/0147-619x(81)90079-2. [DOI] [PubMed] [Google Scholar]
  9. Lovett M. A., Helinski D. R. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid. J Bacteriol. 1976 Aug;127(2):982–987. doi: 10.1128/jb.127.2.982-987.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matsubara K., Otsuji Y. Preparation of plasmids from lambdoid phages and studies on their incompatibilities. Plasmid. 1978 Jun;1(3):284–296. doi: 10.1016/0147-619x(78)90046-x. [DOI] [PubMed] [Google Scholar]
  11. Murotsu T., Matsubara K., Sugisaki H., Takanami M. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid. Gene. 1981 Nov;15(2-3):257–271. doi: 10.1016/0378-1119(81)90135-9. [DOI] [PubMed] [Google Scholar]
  12. Murotsu T., Tsutsui H., Matsubara K. Identification of the minimal essential region for the replication origin of miniF plasmid. Mol Gen Genet. 1984;196(2):373–378. doi: 10.1007/BF00328075. [DOI] [PubMed] [Google Scholar]
  13. Scott J. R. Regulation of plasmid replication. Microbiol Rev. 1984 Mar;48(1):1–23. doi: 10.1016/b978-0-12-048850-6.50006-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Timmis K., Cabello F., Cohen S. N. Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242–2246. doi: 10.1073/pnas.72.6.2242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tsutsui H., Matsubara K. Replication control and switch-off function as observed with a mini-F factor plasmid. J Bacteriol. 1981 Aug;147(2):509–516. doi: 10.1128/jb.147.2.509-516.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES