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. 1994 Jan 1;179(1):57–62. doi: 10.1084/jem.179.1.57

Rat T cell response to superantigens. I. V beta-restricted clonal deletion of rat T cells differentiating in rat-->mouse chimeras

PMCID: PMC2191323  PMID: 8270883

Abstract

T cells of mice display V beta-specific reactivity for a spectrum of mouse mammary tumor virus (Mtv) antigens; confrontation with these antigens during ontogeny causes substantial "holes" in the T cell repertoire. Since endogenous Mtv antigens are rare in other species, the question arises whether V beta-specific recognition of Mtv antigens is unique to mice. To examine this question, rat T cells were allowed to differentiate from stem cells in severe combined immunodeficiency (SCID) mice. These rat-->mouse xenochimeras were prepared under a variety of conditions. The results show that rat T cells are strongly reactive to mouse Mtv antigens, both in terms of tolerogenicity and immunogenicity. In fact, the V beta specificity of rat and mouse T cells for Mtv antigens is almost indistinguishable.

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Selected References

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  1. Acha-Orbea H., Palmer E. Mls--a retrovirus exploits the immune system. Immunol Today. 1991 Oct;12(10):356–361. doi: 10.1016/0167-5699(91)90066-3. [DOI] [PubMed] [Google Scholar]
  2. Baccala R., Kono D. H., Walker S., Balderas R. S., Theofilopoulos A. N. Genomically imposed and somatically modified human thymocyte V beta gene repertoires. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2908–2912. doi: 10.1073/pnas.88.7.2908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baccala R., Vandekerckhove B. A., Jones D., Kono D. H., Roncarolo M. G., Theofilopoulos A. N. Bacterial superantigens mediate T cell deletions in the mouse severe combined immunodeficiency-human liver/thymus model. J Exp Med. 1993 May 1;177(5):1481–1485. doi: 10.1084/jem.177.5.1481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brodsky F. M., Guagliardi L. E. The cell biology of antigen processing and presentation. Annu Rev Immunol. 1991;9:707–744. doi: 10.1146/annurev.iy.09.040191.003423. [DOI] [PubMed] [Google Scholar]
  5. Cazenave P. A., Marche P. N., Jouvin-Marche E., Voegtlé D., Bonhomme F., Bandeira A., Coutinho A. V beta 17 gene polymorphism in wild-derived mouse strains: two amino acid substitutions in the V beta 17 region greatly alter T cell receptor specificity. Cell. 1990 Nov 16;63(4):717–728. doi: 10.1016/0092-8674(90)90138-5. [DOI] [PubMed] [Google Scholar]
  6. Frey J. R., Ernst B., Surh C. D., Sprent J. Thymus-grafted SCID mice show transient thymopoiesis and limited depletion of V beta 11+ T cells. J Exp Med. 1992 Apr 1;175(4):1067–1071. doi: 10.1084/jem.175.4.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gold D. P., Vainiene M., Celnik B., Wiley S., Gibbs C., Hashim G. A., Vandenbark A. A., Offner H. Characterization of the immune response to a secondary encephalitogenic epitope of basic protein in Lewis rats. II. Biased T cell receptor V beta expression predominates in spinal cord infiltrating T cells. J Immunol. 1992 Mar 15;148(6):1712–1717. [PubMed] [Google Scholar]
  8. Golovkina T. V., Chervonsky A., Dudley J. P., Ross S. R. Transgenic mouse mammary tumor virus superantigen expression prevents viral infection. Cell. 1992 May 15;69(4):637–645. doi: 10.1016/0092-8674(92)90227-4. [DOI] [PubMed] [Google Scholar]
  9. Janeway C. A., Jr, Yagi J., Conrad P. J., Katz M. E., Jones B., Vroegop S., Buxser S. T-cell responses to Mls and to bacterial proteins that mimic its behavior. Immunol Rev. 1989 Feb;107:61–88. doi: 10.1111/j.1600-065x.1989.tb00003.x. [DOI] [PubMed] [Google Scholar]
  10. Kampinga J., Kroese F. G., Pol G. H., Nieuwenhuis P., Haag F., Singh P. B., Roser B., Aspinall R. A monoclonal antibody to a determinant of the rat T cell antigen receptor expressed by a minor subset of T cells. Int Immunol. 1989;1(3):289–295. doi: 10.1093/intimm/1.3.289. [DOI] [PubMed] [Google Scholar]
  11. Kappler J. W., Roehm N., Marrack P. T cell tolerance by clonal elimination in the thymus. Cell. 1987 Apr 24;49(2):273–280. doi: 10.1016/0092-8674(87)90568-x. [DOI] [PubMed] [Google Scholar]
  12. Labrecque N., McGrath H., Subramanyam M., Huber B. T., Sékaly R. P. Human T cells respond to mouse mammary tumor virus-encoded superantigen: V beta restriction and conserved evolutionary features. J Exp Med. 1993 Jun 1;177(6):1735–1743. doi: 10.1084/jem.177.6.1735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pullen A. M., Bill J., Kubo R. T., Marrack P., Kappler J. W. Analysis of the interaction site for the self superantigen Mls-1a on T cell receptor V beta. J Exp Med. 1991 May 1;173(5):1183–1192. doi: 10.1084/jem.173.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pullen A. M., Wade T., Marrack P., Kappler J. W. Identification of the region of T cell receptor beta chain that interacts with the self-superantigen MIs-1a. Cell. 1990 Jun 29;61(7):1365–1374. doi: 10.1016/0092-8674(90)90700-o. [DOI] [PubMed] [Google Scholar]
  15. Smith L. R., Kono D. H., Kammuller M. E., Balderas R. S., Theofilopoulos A. N. V beta repertoire in rats and implications for endogenous superantigens. Eur J Immunol. 1992 Mar;22(3):641–645. doi: 10.1002/eji.1830220305. [DOI] [PubMed] [Google Scholar]
  16. Smith L. R., Kono D. H., Theofilopoulos A. N. Complexity and sequence identification of 24 rat V beta genes. J Immunol. 1991 Jul 1;147(1):375–379. [PubMed] [Google Scholar]
  17. Sprent J., Schaefer M. Properties of purified T cell subsets. I. In vitro responses to class I vs. class II H-2 alloantigens. J Exp Med. 1985 Dec 1;162(6):2068–2088. doi: 10.1084/jem.162.6.2068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Surh C. D., Sprent J. Long-term xenogeneic chimeras. Full differentiation of rat T and B cells in SCID mice. J Immunol. 1991 Oct 1;147(7):2148–2154. [PubMed] [Google Scholar]
  19. Torres-Nagel N. E., Gold D. P., Hünig T. Identification of rat Tcrb-V8.2, 8.5, and 10 gene products by monoclonal antibodies. Immunogenetics. 1993;37(4):305–308. doi: 10.1007/BF00187460. [DOI] [PubMed] [Google Scholar]
  20. Widmer M. B., Bach F. H. Allogeneic and xenogeneic response in mixed leukocyte cultures. J Exp Med. 1972 May 1;135(5):1204–1208. doi: 10.1084/jem.135.5.1204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zhang X. M., Heber-Katz E. T cell receptor sequences from encephalitogenic T cells in adult Lewis rats suggest an early ontogenic origin. J Immunol. 1992 Feb 1;148(3):746–752. [PubMed] [Google Scholar]

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