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. 1985 Aug;163(2):792–795. doi: 10.1128/jb.163.2.792-795.1985

Localization of the enantiozymes of 6-hydroxy-nicotine oxidase in Arthrobacter oxidans by electron immunochemistry.

J R Swafford, H C Reeves, R Brandsch
PMCID: PMC219194  PMID: 4019415

Abstract

During the course of growth of Arthrobacter oxidans, induction of the enantiozymes 6-hydroxy-D-nicotine oxidase and 6-hydroxy-L-nicotine oxidase occurred in the presence of DL-nicotine. Cryoultramicrotomed sections obtained from cells grown to stationary phase were gold immunolabeled. The results obtained demonstrate that both enzymes are localized in the cytoplasm.

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Selected References

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  1. Decker K., Bleeg H. Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans. Biochim Biophys Acta. 1965 Aug 24;105(2):313–324. doi: 10.1016/s0926-6593(65)80155-2. [DOI] [PubMed] [Google Scholar]
  2. Decker K., Dai V. D. Mechanism and specifcity of L- and D-6-hydroxynicotine oxidase. Eur J Biochem. 1967 Dec;3(2):132–138. doi: 10.1111/j.1432-1033.1967.tb19507.x. [DOI] [PubMed] [Google Scholar]
  3. Decker K., Dai V. D., Möhler H., Brühmüller M. D- and L-6-hydroxynicotine oxidase, enantiozymes of Arthrobacter oxidans. Z Naturforsch B. 1972 Sep;27(9):1072–1073. doi: 10.1515/znb-1972-0921. [DOI] [PubMed] [Google Scholar]
  4. EBERWEIN H., GRIES F. A., DECKER K. [On the decomposition of nicotine by bacterial enzymes. II. Isolation and characterization of a nicotine-splitting soil bacterium]. Hoppe Seylers Z Physiol Chem. 1961 May 3;323:236–248. doi: 10.1515/bchm2.1961.323.1.236. [DOI] [PubMed] [Google Scholar]
  5. Gloger M., Decker K. Zum Mechanismus der Induktion nicotinabbauender Enzyme in Arthrobacter oxydans. Z Naturforsch B. 1969 Aug;24(8):1016–1025. [PubMed] [Google Scholar]
  6. Möhler H., Brühmüller M., Decker K. Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Identification of the 8 -(N-3-histidyl)-riboflavin-linkage between FAD and apoenzyme. Eur J Biochem. 1972 Aug 18;29(1):152–155. doi: 10.1111/j.1432-1033.1972.tb01969.x. [DOI] [PubMed] [Google Scholar]
  7. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  9. Swafford J. R., Malloy P. J., Reeves H. C. Immunochemical localization of NADP-specific isocitrate dehydrogenase in Escherichia coli. Science. 1983 Jul 15;221(4607):295–296. doi: 10.1126/science.6344223. [DOI] [PubMed] [Google Scholar]

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