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. 1985 Sep;163(3):913–917. doi: 10.1128/jb.163.3.913-917.1985

Mu-lac insertion-directed mutagenesis in a pectate lyase gene of Erwinia chrysanthemi.

A Diolez, A Coleno
PMCID: PMC219219  PMID: 2993251

Abstract

The pelC gene, which encodes one of the five major pectate lyase (PL) isoenzymes in Erwinia chrysanthemi 3937, designated PLc, was subcloned from a hybrid lambda phage into a pBR322 derivative and mutagenized with a mini-Mu-lacZ transposable element able to form fusions to the lacZ gene. One plasmid (pAD1) which had an inactivated pelC gene and a Lac+ phenotype was selected in Escherichia coli. This plasmid was introduced into Erwinia chrysanthemi, and the pelC::mini-Mu insertion was substituted for the chromosomal allele by homologous recombination. This strain lacks the PLc isoenzyme. This Erwinia chrysanthemi strain has a Lac+ phenotype that is inducible by polygalacturonate, as are the wild-type PL activities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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