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. 1995 Nov 1;182(5):1603–1608. doi: 10.1084/jem.182.5.1603

CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73

PMCID: PMC2192217  PMID: 7595232

Abstract

We have recently described a monoclonal antibody (mAb) 4G4 recognizing a 70-kD molecule constitutively expressed on human endothelial cells and on subpopulations of lymphocytes. We showed that this molecule, which we named lymphocyte-vascular adhesion protein 2 (L-VAP-2), mediates lymphocyte adhesion to cultured endothelial cells. Protein sequencing of tryptic peptides from immunoaffinity-purified L-VAP-2 revealed sequence identity between L-VAP-2 and CD73 (ecto-5'- nucleotidase, E.C.3.1.3.5), and COS cells transfected with a CD73 cDNA were positively stained with the mAb 4G4, which recognizes L-VAP-2. mAb 4G4 was also able to partially inhibit the ecto-5'-nucleotidase activity of peripheral blood lymphocytes. Moreover, cross-precipitation studies performed with mAb 4G4 and a CD73 workshop mAb 1E9 showed that these two antibodies recognize the same molecule. Since the tissue distribution and biochemical characteristics of the two molecules are also similar, we conclude that L-VAP-2 and CD73 are the same glycoprotein. Adhesion experiments showed significantly increased binding of freshly isolated lymphocytes to COS cells transfected with a CD73 cDNA, as compared to mock-transfected COS cells, and binding of lymphocytes to CD73-expressing COS cells was inhibited by the presence of mAb 4G4 in the adhesion assay. CD73 is a glycosyl phosphatidylinositol-linked molecule previously shown to have a cosignalling role in T lymphocyte proliferation. Our data suggest that it also has a function in mediating lymphocyte adhesion to the endothelium.

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Selected References

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  1. Airas L., Salmi M., Jalkanen S. Lymphocyte-vascular adhesion protein-2 is a novel 70-kDa molecule involved in lymphocyte adhesion to vascular endothelium. J Immunol. 1993 Oct 15;151(8):4228–4238. [PubMed] [Google Scholar]
  2. Bastian J. F., Ruedi J. M., MacPherson G. A., Golembesky H. E., O'Connor R. D., Thompson L. F. Lymphocyte ecto-5'-nucleotidase activity in infancy: increasing activity in peripheral blood B cells precedes their ability to synthesize IgG in vitro. J Immunol. 1984 Apr;132(4):1767–1772. [PubMed] [Google Scholar]
  3. Cronstein B. N., Levin R. I., Philips M., Hirschhorn R., Abramson S. B., Weissmann G. Neutrophil adherence to endothelium is enhanced via adenosine A1 receptors and inhibited via adenosine A2 receptors. J Immunol. 1992 Apr 1;148(7):2201–2206. [PubMed] [Google Scholar]
  4. Dianzani U., Redoglia V., Bragardo M., Attisano C., Bianchi A., Di Franco D., Ramenghi U., Wolff H., Thompson L. F., Pileri A. Co-stimulatory signal delivered by CD73 molecule to human CD45RAhiCD45ROlo (naive) CD8+ T lymphocytes. J Immunol. 1993 Oct 15;151(8):3961–3970. [PubMed] [Google Scholar]
  5. Edwards N. L., Magilavy D. B., Cassidy J. T., Fox I. H. Lymphocyte ecto-5'-nucleotidase deficiency in agammaglobulinemia. Science. 1978 Aug 18;201(4356):628–630. doi: 10.1126/science.27864. [DOI] [PubMed] [Google Scholar]
  6. Fernandez J., DeMott M., Atherton D., Mische S. M. Internal protein sequence analysis: enzymatic digestion for less than 10 micrograms of protein bound to polyvinylidene difluoride or nitrocellulose membranes. Anal Biochem. 1992 Mar;201(2):255–264. doi: 10.1016/0003-2697(92)90336-6. [DOI] [PubMed] [Google Scholar]
  7. Gelfand E. W., McCurdy D., Rao C. P., Cohen A. Absence of lymphocyte ecto-5'-nucleotidase in infants with reticuloendotheliosis and eosinophilia (Omenn's syndrome). Blood. 1984 Jun;63(6):1475–1480. [PubMed] [Google Scholar]
  8. Hynes R. O. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992 Apr 3;69(1):11–25. doi: 10.1016/0092-8674(92)90115-s. [DOI] [PubMed] [Google Scholar]
  9. Jalkanen S., Jalkanen M., Bargatze R., Tammi M., Butcher E. C. Biochemical properties of glycoproteins involved in lymphocyte recognition of high endothelial venules in man. J Immunol. 1988 Sep 1;141(5):1615–1623. [PubMed] [Google Scholar]
  10. Lasky L. A. Selectins: interpreters of cell-specific carbohydrate information during inflammation. Science. 1992 Nov 6;258(5084):964–969. doi: 10.1126/science.1439808. [DOI] [PubMed] [Google Scholar]
  11. Massaia M., Perrin L., Bianchi A., Ruedi J., Attisano C., Altieri D., Rijkers G. T., Thompson L. F. Human T cell activation. Synergy between CD73 (ecto-5'-nucleotidase) and signals delivered through CD3 and CD2 molecules. J Immunol. 1990 Sep 15;145(6):1664–1674. [PubMed] [Google Scholar]
  12. Misumi Y., Ogata S., Ohkubo K., Hirose S., Ikehara Y. Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form. Eur J Biochem. 1990 Aug 17;191(3):563–569. doi: 10.1111/j.1432-1033.1990.tb19158.x. [DOI] [PubMed] [Google Scholar]
  13. Sako D., Chang X. J., Barone K. M., Vachino G., White H. M., Shaw G., Veldman G. M., Bean K. M., Ahern T. J., Furie B. Expression cloning of a functional glycoprotein ligand for P-selectin. Cell. 1993 Dec 17;75(6):1179–1186. doi: 10.1016/0092-8674(93)90327-m. [DOI] [PubMed] [Google Scholar]
  14. Salazar-Gonzalez J. F., Moody D. J., Giorgi J. V., Martinez-Maza O., Mitsuyasu R. T., Fahey J. L. Reduced ecto-5'-nucleotidase activity and enhanced OKT10 and HLA-DR expression on CD8 (T suppressor/cytotoxic) lymphocytes in the acquired immune deficiency syndrome: evidence of CD8 cell immaturity. J Immunol. 1985 Sep;135(3):1778–1785. [PubMed] [Google Scholar]
  15. Springer T. A. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994 Jan 28;76(2):301–314. doi: 10.1016/0092-8674(94)90337-9. [DOI] [PubMed] [Google Scholar]
  16. Steegmaier M., Levinovitz A., Isenmann S., Borges E., Lenter M., Kocher H. P., Kleuser B., Vestweber D. The E-selectin-ligand ESL-1 is a variant of a receptor for fibroblast growth factor. Nature. 1995 Feb 16;373(6515):615–620. doi: 10.1038/373615a0. [DOI] [PubMed] [Google Scholar]
  17. Thompson L. F., O'Connor R. D., Bastian J. F. Phenotype and function of engrafted maternal T cells in patients with severe combined immunodeficiency. J Immunol. 1984 Nov;133(5):2513–2517. [PubMed] [Google Scholar]
  18. Thompson L. F., Ruedi J. M., Glass A., Low M. G., Lucas A. H. Antibodies to 5'-nucleotidase (CD73), a glycosyl-phosphatidylinositol-anchored protein, cause human peripheral blood T cells to proliferate. J Immunol. 1989 Sep 15;143(6):1815–1821. [PubMed] [Google Scholar]
  19. Thompson L. F., Ruedi J. M., O'Connor R. D., Bastian J. F. Ecto-5'-nucleotidase expression during human B cell development. An explanation for the heterogeneity in B lymphocyte ecto-5'-nucleotidase activity in patients with hypogammaglobulinemia. J Immunol. 1986 Oct 15;137(8):2496–2500. [PubMed] [Google Scholar]
  20. Thomson L. F., Ruedi J. M., Glass A., Moldenhauer G., Moller P., Low M. G., Klemens M. R., Massaia M., Lucas A. H. Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73). Tissue Antigens. 1990 Jan;35(1):9–19. doi: 10.1111/j.1399-0039.1990.tb01750.x. [DOI] [PubMed] [Google Scholar]

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