Abstract
Vpre-B and lambda 5 genes, respectively, encode V-like and C-like domains of a surrogate immunoglobulin light chain (psi L). Such psi L complex is expressed in early progenitor B (pro-B) cells, before conventional immunoglobulin heavy (microH) and light (L) chains are produced. We raised a wide panel of monoclonal antibodies (mAbs) against soluble recombinant Vpre-B proteins to study early events in human B cell development. One of these antibodies, B-MAD688, labeled surrogate Ig-complexes on the surface of microH- pro-B cell lines and normal bone marrow cells in immunofluorescence assays. Immunoprecipitations using surface-labeled pro-B cells and B-MAD688 mAb indicated that human psi L is associated with high molecular weight components homologous to the surrogate heavy (psi H) chains described in mouse. Using B-MAD688 and SLC2 mAbs, we were able to distinguish between psi H psi L and microH psi L complexes on the surface of human pro-B and later precursor, pre-B, cells. The finding of psi H psi L complexes in mouse and man lead us to hypothesize a role for psi H- containing receptors in B cell development.
Full Text
The Full Text of this article is available as a PDF (748.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauer S. R., Kudo A., Melchers F. Structure and pre-B lymphocyte restricted expression of the VpreB in humans and conservation of its structure in other mammalian species. EMBO J. 1988 Jan;7(1):111–116. doi: 10.1002/j.1460-2075.1988.tb02789.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ehlich A., Schaal S., Gu H., Kitamura D., Müller W., Rajewsky K. Immunoglobulin heavy and light chain genes rearrange independently at early stages of B cell development. Cell. 1993 Mar 12;72(5):695–704. doi: 10.1016/0092-8674(93)90398-a. [DOI] [PubMed] [Google Scholar]
- Guelpa-Fonlupt V., Tonnelle C., Blaise D., Fougereau M., Fumoux F. Discrete early pro-B and pre-B stages in normal human bone marrow as defined by surface pseudo-light chain expression. Eur J Immunol. 1994 Jan;24(1):257–264. doi: 10.1002/eji.1830240140. [DOI] [PubMed] [Google Scholar]
- Iglesias A., Kopf M., Williams G. S., Bühler B., Köhler G. Molecular requirements for the mu-induced light chain gene rearrangement in pre-B cells. EMBO J. 1991 Aug;10(8):2147–2155. doi: 10.1002/j.1460-2075.1991.tb07749.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karasuyama H., Rolink A., Melchers F. A complex of glycoproteins is associated with VpreB/lambda 5 surrogate light chain on the surface of mu heavy chain-negative early precursor B cell lines. J Exp Med. 1993 Aug 1;178(2):469–478. doi: 10.1084/jem.178.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karasuyama H., Rolink A., Shinkai Y., Young F., Alt F. W., Melchers F. The expression of Vpre-B/lambda 5 surrogate light chain in early bone marrow precursor B cells of normal and B cell-deficient mutant mice. Cell. 1994 Apr 8;77(1):133–143. doi: 10.1016/0092-8674(94)90241-0. [DOI] [PubMed] [Google Scholar]
- Kitamura D., Kudo A., Schaal S., Müller W., Melchers F., Rajewsky K. A critical role of lambda 5 protein in B cell development. Cell. 1992 May 29;69(5):823–831. doi: 10.1016/0092-8674(92)90293-l. [DOI] [PubMed] [Google Scholar]
- Kubagawa H., Cooper M. D., Carroll A. J., Burrows P. D. Light-chain gene expression before heavy-chain gene rearrangement in pre-B cells transformed by Epstein-Barr virus. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2356–2360. doi: 10.1073/pnas.86.7.2356. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lassoued K., Nuñez C. A., Billips L., Kubagawa H., Monteiro R. C., LeBlen T. W., Cooper M. D. Expression of surrogate light chain receptors is restricted to a late stage in pre-B cell differentiation. Cell. 1993 Apr 9;73(1):73–86. doi: 10.1016/0092-8674(93)90161-i. [DOI] [PubMed] [Google Scholar]
- Marcos M. A., Godin I., Cumano A., Morales S., Garcia-Porrero J. A., Dieterlen-Lievre F., Gaspar M. L. Developmental events from hemopoietic stem cells to B-cell populations and Ig repertoires. Immunol Rev. 1994 Feb;137:155–171. doi: 10.1111/j.1600-065x.1994.tb00663.x. [DOI] [PubMed] [Google Scholar]
- Rolink A., Karasuyama H., Haasner D., Grawunder U., Mårtensson I. L., Kudo A., Melchers F. Two pathways of B-lymphocyte development in mouse bone marrow and the roles of surrogate L chain in this development. Immunol Rev. 1994 Feb;137:185–201. doi: 10.1111/j.1600-065x.1994.tb00665.x. [DOI] [PubMed] [Google Scholar]
- Shinjo F., Hardy R. R., Jongstra J. Monoclonal anti-lambda 5 antibody FS1 identifies a 130 kDa protein associated with lambda 5 and Vpre-B on the surface of early pre-B cell lines. Int Immunol. 1994 Mar;6(3):393–399. doi: 10.1093/intimm/6.3.393. [DOI] [PubMed] [Google Scholar]
- Traunecker A., Schneider J., Kiefer H., Karjalainen K. Highly efficient neutralization of HIV with recombinant CD4-immunoglobulin molecules. Nature. 1989 May 4;339(6219):68–70. doi: 10.1038/339068a0. [DOI] [PubMed] [Google Scholar]
- Tsubata T., Tsubata R., Reth M. Crosslinking of the cell surface immunoglobulin (mu-surrogate light chains complex) on pre-B cells induces activation of V gene rearrangements at the immunoglobulin kappa locus. Int Immunol. 1992 Jun;4(6):637–641. doi: 10.1093/intimm/4.6.637. [DOI] [PubMed] [Google Scholar]
- Tunnacliffe A., Olsson C., de la Hera A. The majority of human CD3 epitopes are conferred by the epsilon chain. Int Immunol. 1989;1(5):546–550. doi: 10.1093/intimm/1.5.546. [DOI] [PubMed] [Google Scholar]
- Winkler T. H., Rolink A., Melchers F., Karasuyama H. Precursor B cells of mouse bone marrow express two different complexes with the surrogate light chain on the surface. Eur J Immunol. 1995 Feb;25(2):446–450. doi: 10.1002/eji.1830250221. [DOI] [PubMed] [Google Scholar]
- ten Boekel E., Melchers F., Rolink A. The status of Ig loci rearrangements in single cells from different stages of B cell development. Int Immunol. 1995 Jun;7(6):1013–1019. doi: 10.1093/intimm/7.6.1013. [DOI] [PubMed] [Google Scholar]