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. 1985 Dec;164(3):977–982. doi: 10.1128/jb.164.3.977-982.1985

Nucleotide sequence of the gene determining plasmid-mediated citrate utilization.

N Ishiguro, G Sato
PMCID: PMC219287  PMID: 2999087

Abstract

The citrate utilization determinant from transposon Tn3411 has been cloned and sequenced, and its polypeptide products have been characterized in minicell experiments. The nucleotide sequence was determined for a 2,047-base-pair BglII restriction endonuclease fragment that includes the citrate determinant. This region contains an open reading frame that would encode a 431-amino-acid very hydrophobic polypeptide and which is preceded by a reasonable ribosomal binding site. However, the single polypeptide found in minicell experiments had an apparent molecular weight of 35,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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Selected References

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