Abstract
Cells of the cyanobacterium Nostoc commune UTEX 584 in exponential growth were subjected to acute water stress by immobilizing them on solid supports and drying them at a matric water potential (psi m) of -99.5 MPa. Cells which had been grown in the presence of Na235SO4 before immobilization and rapid drying continued to incorporate 35S into protein for 90 min. This incorporation was inhibited by chloramphenicol. No unique proteins appeared to be synthesized during this time. Upon further drying, the level of incorporation of 35S in protein began to decrease. In contrast, there was an apparent increase in the level of certain phycobiliprotein subunits in solubilized protein extracts of these cells. Extensive proteolysis was detected after prolonged desiccation (17 days) of the cells in the light, although they still remained intact. Phycobilisomes became dissociated in both light- and dark-stored desiccated material.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Biessmann H., Levy B., McCarthy B. J. In vitro transcription of heat-shock-specific RNA from chromatin of Drosophila melanogaster cells. Proc Natl Acad Sci U S A. 1978 Feb;75(2):759–763. doi: 10.1073/pnas.75.2.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Borbély G., Surányi G., Korcz A., Pálfi Z. Effect of heat shock on protein synthesis in the cyanobacterium Synechococcus sp. strain PCC 6301. J Bacteriol. 1985 Mar;161(3):1125–1130. doi: 10.1128/jb.161.3.1125-1130.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Borowitzka L. J., Demmerle S., Mackay M. A., Norton R. S. Carbon-13 nuclear magnetic resonance study of osmoregulation in a blue-green alga. Science. 1980 Nov 7;210(4470):650–651. doi: 10.1126/science.210.4470.650. [DOI] [PubMed] [Google Scholar]
- Collins P. L., Hightower L. E. Newcastle disease virus stimulates the cellular accumulation of stress (heat shock) mRNAs and proteins. J Virol. 1982 Nov;44(2):703–707. doi: 10.1128/jvi.44.2.703-707.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daniels C. J., McKee A. H., Doolittle W. F. Archaebacterial heat-shock proteins. EMBO J. 1984 Apr;3(4):745–749. doi: 10.1002/j.1460-2075.1984.tb01878.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lugtenberg B., Peters R., Bernheimer H., Berendsen W. Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli. Mol Gen Genet. 1976 Sep 23;147(3):251–262. doi: 10.1007/BF00582876. [DOI] [PubMed] [Google Scholar]
- Neidhardt F. C., VanBogelen R. A., Lau E. T. Molecular cloning and expression of a gene that controls the high-temperature regulon of Escherichia coli. J Bacteriol. 1983 Feb;153(2):597–603. doi: 10.1128/jb.153.2.597-603.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Norton R. S., MacKay M. A., Borowitzka L. J. The physical state of osmoregulatory solutes in unicellular algae. A natural-abundance carbon-13 nuclear-magnetic-resonance relaxation study. Biochem J. 1982 Mar 15;202(3):699–706. doi: 10.1042/bj2020699. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oliver M. J., Bewley J. D. Plant Desiccation and Protein Synthesis : V. Stability of Poly (A) and Poly (A) RNA during Desiccation and Their Synthesis upon Rehydration in the Desiccation-Tolerant Moss Tortula ruralis and the Intolerant Moss Cratoneuron filicinum. Plant Physiol. 1984 Apr;74(4):917–922. doi: 10.1104/pp.74.4.917. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oliver M. J., Bewley J. D. Plant Desiccation and Protein Synthesis : VI. Changes in Protein Synthesis Elicited by Desiccation of the Moss Tortula ruralis are Effected at the Translational Level. Plant Physiol. 1984 Apr;74(4):923–927. doi: 10.1104/pp.74.4.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart R. R., Bewley J. D. Stability and Synthesis of Phospholipids during Desiccation and Rehydration of a Desiccation-Tolerant and a Desiccation-Intolerant Moss. Plant Physiol. 1982 Mar;69(3):724–727. doi: 10.1104/pp.69.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White C. N., Hightower L. E. Stress mRNA metabolism in canavanine-treated chicken embryo cells. Mol Cell Biol. 1984 Aug;4(8):1534–1541. doi: 10.1128/mcb.4.8.1534. [DOI] [PMC free article] [PubMed] [Google Scholar]