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. 1985 Dec;164(3):1032–1038. doi: 10.1128/jb.164.3.1032-1038.1985

Glutamine synthetase-constitutive mutation affecting the glnALG upstream promoter of Escherichia coli.

P León, D Romero, A Garciarrubio, F Bastarrachea, A A Covarrubias
PMCID: PMC219294  PMID: 2866175

Abstract

The spontaneous gln-76 mutation of Escherichia coli (Osorio et al., Mol. Gen. Genet. 194:114-123, 1984) was previously shown to be responsible for the cis-dominant constitutive expression of the glnA gene in the absence of a glnG-glnF activator system. Nucleotide sequence analysis has now revealed that gln-76 is a single transversion T.A to A.T, an up-promoter mutation affecting the -10 region of glnAp1, the upstream promoter of the glnALG control region. Both, wild-type and gln-76 DNA control regions were cloned into the promoter-probe plasmid pKO1. Galactokinase activity determinations of cells carrying the fused plasmids showed 10-fold more effective expression mediated by gln-76 than by the glnA wild-type control region. Primer extension experiments with RNA from strains carrying the gln-76 control region indicated that the transcription initiation sites were the same in both the gln-76 mutant and the wild type.

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Selected References

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