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. 1985 Dec;164(3):1353–1355. doi: 10.1128/jb.164.3.1353-1355.1985

lac Up-promoter mutants with increased homology to the consensus promoter sequence.

W Mandecki, R A Goldman, B S Powell, M H Caruthers
PMCID: PMC219337  PMID: 2999082

Abstract

Four lac promoter mutants were constructed. The mutations increased the homology between the lac promoter and the consensus promoter sequences by introducing the consensus -10 and -35 regions and the consensus spacing of 17 residues between these two regions. The promoter mutants were cloned into a pBR322-derivatized vector upstream from the lacZ gene, and levels of beta-galactosidase were an indication of promoter activity. All mutants exhibited higher activity than did the wild-type promoter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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