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. 1985 Dec;164(3):1370–1372. doi: 10.1128/jb.164.3.1370-1372.1985

Excretion of unassembled hook-associated proteins by Salmonella typhimurium.

M Homma, T Iino
PMCID: PMC219342  PMID: 3905770

Abstract

Hook-associated proteins (HAPs) were excreted into the culture medium of the Fla+ strain as well as into the growth medium of the filamentless mutants of Salmonella typhimurium. This indicates that the bacteria synthesize HAPs excessively, beyond the amount required for construction of flagella. The extra HAPs are shed into the culture medium after a definite amount of each HAP has been assembled into the flagellar structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DePamphilis M. L., Adler J. Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer, lipopolysaccharide membrane and the cyoplasmic membrane. J Bacteriol. 1971 Jan;105(1):396–407. doi: 10.1128/jb.105.1.396-407.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DePamphilis M. L., Adler J. Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis. J Bacteriol. 1971 Jan;105(1):384–395. doi: 10.1128/jb.105.1.384-395.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doetsch R. N., Sjoblad R. D. Flagellar structure and function in eubacteria. Annu Rev Microbiol. 1980;34:69–108. doi: 10.1146/annurev.mi.34.100180.000441. [DOI] [PubMed] [Google Scholar]
  4. Emerson S. U., Tokuyasu K., Simon M. I. Bacterial flagella: polarity of elongation. Science. 1970 Jul 10;169(3941):190–192. doi: 10.1126/science.169.3941.190. [DOI] [PubMed] [Google Scholar]
  5. Fujita H., Yamaguchi S., Taira T., Iino T. A simple method for the isolation of flagellar shape mutants in Salmonella. J Gen Microbiol. 1981 Jul;125(1):213–216. doi: 10.1099/00221287-125-1-213. [DOI] [PubMed] [Google Scholar]
  6. Homma M., Fujita H., Yamaguchi S., Iino T. Excretion of unassembled flagellin by Salmonella typhimurium mutants deficient in hook-associated proteins. J Bacteriol. 1984 Sep;159(3):1056–1059. doi: 10.1128/jb.159.3.1056-1059.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Homma M., Iino T. Locations of hook-associated proteins in flagellar structures of Salmonella typhimurium. J Bacteriol. 1985 Apr;162(1):183–189. doi: 10.1128/jb.162.1.183-189.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Homma M., Kutsukake K., Iino T. Structural genes for flagellar hook-associated proteins in Salmonella typhimurium. J Bacteriol. 1985 Aug;163(2):464–471. doi: 10.1128/jb.163.2.464-471.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Homma M., Kutsukake K., Iino T., Yamaguchi S. Hook-associated proteins essential for flagellar filament formation in Salmonella typhimurium. J Bacteriol. 1984 Jan;157(1):100–108. doi: 10.1128/jb.157.1.100-108.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Iino T. Assembly of Salmonella flagellin in vitro and in vivo. J Supramol Struct. 1974;2(2-4):372–384. doi: 10.1002/jss.400020226. [DOI] [PubMed] [Google Scholar]
  11. Iino T. Polarity of flagellar growth in salmonella. J Gen Microbiol. 1969 May;56(2):227–239. doi: 10.1099/00221287-56-2-227. [DOI] [PubMed] [Google Scholar]
  12. Kagawa H., Nishiyama T., Yamaguchi S. Motility development of Salmonella typhimurium cells with flaV mutations after addition of exogenous flagellin. J Bacteriol. 1983 Jul;155(1):435–437. doi: 10.1128/jb.155.1.435-437.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. O'Brien E. J., Bennett P. M. Structure of straight flagella from a mutant Salmonella. J Mol Biol. 1972 Sep 14;70(1):133–152. doi: 10.1016/0022-2836(72)90168-4. [DOI] [PubMed] [Google Scholar]
  15. Shirakihara Y., Wakabayashi T. Three-dimensional image reconstruction of straight flagella from a mutant Salmonella typhimurium. J Mol Biol. 1979 Jul 5;131(3):485–507. doi: 10.1016/0022-2836(79)90004-4. [DOI] [PubMed] [Google Scholar]
  16. Suzuki T., Iino T., Horiguchi T., Yamaguchi S. Incomplete flagellar structures in nonflagellate mutants of Salmonella typhimurium. J Bacteriol. 1978 Feb;133(2):904–915. doi: 10.1128/jb.133.2.904-915.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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