Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Dec;164(3):1373–1375. doi: 10.1128/jb.164.3.1373-1375.1985

Transfer of DNA killer plasmids from Kluyveromyces lactis to Kluyveromyces fragilis and Candida pseudotropicalis.

Y Sugisaki, N Gunge, K Sakaguchi, M Yamasaki, G Tamura
PMCID: PMC219343  PMID: 4066615

Abstract

Killer plasmids pGKL1 and pGKL2 of double-stranded linear DNAs were transferred from Kluyveromyces lactis to strains of Kluyveromyces fragilis and Candida pseudotropicalis. The resultant killer strains produced 17-fold and 6-fold larger amounts of killer toxin than K. lactis did, respectively. The killer toxin produced by each species appeared to be a glycoprotein.

Full text

PDF
1373

Images in this article

1374Image
on p.1374

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bostian K. A., Jayachandran S., Tipper D. J. A glycosylated protoxin in killer yeast: models for its structure and maturation. Cell. 1983 Jan;32(1):169–180. doi: 10.1016/0092-8674(83)90507-x. [DOI] [PubMed] [Google Scholar]
  2. Bussey H. Physiology of killer factor in yeast. Adv Microb Physiol. 1981;22:93–122. doi: 10.1016/s0065-2911(08)60326-4. [DOI] [PubMed] [Google Scholar]
  3. Bussey H., Saville D., Greene D., Tipper D. J., Bostian K. A. Secretion of Saccharomyces cerevisiae killer toxin: processing of the glycosylated precursor. Mol Cell Biol. 1983 Aug;3(8):1362–1370. doi: 10.1128/mcb.3.8.1362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gunge N., Murata K., Sakaguchi K. Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis. J Bacteriol. 1982 Jul;151(1):462–464. doi: 10.1128/jb.151.1.462-464.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gunge N., Sakaguchi K. Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKl1 and pGKl2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion. J Bacteriol. 1981 Jul;147(1):155–160. doi: 10.1128/jb.147.1.155-160.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gunge N., Tamaru A., Ozawa F., Sakaguchi K. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. J Bacteriol. 1981 Jan;145(1):382–390. doi: 10.1128/jb.145.1.382-390.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hishinuma F., Nakamura K., Hirai K., Nishizawa R., Gunge N., Maeda T. Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast. Nucleic Acids Res. 1984 Oct 11;12(19):7581–7597. doi: 10.1093/nar/12.19.7581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Julius D., Schekman R., Thorner J. Glycosylation and processing of prepro-alpha-factor through the yeast secretory pathway. Cell. 1984 Feb;36(2):309–318. doi: 10.1016/0092-8674(84)90224-1. [DOI] [PubMed] [Google Scholar]
  9. Niwa O., Sakaguchi K., Gunge N. Curing of the killer deoxyribonucleic acid plasmids of Kluyveromyces lactis. J Bacteriol. 1981 Dec;148(3):988–990. doi: 10.1128/jb.148.3.988-990.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stark M. J., Mileham A. J., Romanos M. A., Boyd A. Nucleotide sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeast Kluyveromyces lactis. Nucleic Acids Res. 1984 Aug 10;12(15):6011–6030. doi: 10.1093/nar/12.15.6011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sugisaki Y., Gunge N., Sakaguchi K., Yamasaki M., Tamura G. Characterization of a novel killer toxin encoded by a double-stranded linear DNA plasmid of Kluyveromyces lactis. Eur J Biochem. 1984 Jun 1;141(2):241–245. doi: 10.1111/j.1432-1033.1984.tb08183.x. [DOI] [PubMed] [Google Scholar]
  12. Sugisaki Y., Gunge N., Sakaguchi K., Yamasaki M., Tamura G. Kluyveromyces lactis killer toxin inhibits adenylate cyclase of sensitive yeast cells. Nature. 1983 Aug 4;304(5925):464–466. doi: 10.1038/304464a0. [DOI] [PubMed] [Google Scholar]
  13. Tipper D. J., Bostian K. A. Double-stranded ribonucleic acid killer systems in yeasts. Microbiol Rev. 1984 Jun;48(2):125–156. doi: 10.1128/mr.48.2.125-156.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WICKERHAM L. J., BURTON K. A. Hybridization studies involving Saccharomyces lactis and Zygosaccharomyces ashbyi. J Bacteriol. 1956 Mar;71(3):290–295. doi: 10.1128/jb.71.3.290-295.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES