Abstract
The components of the proton motive force (delta p), namely the membrane potential and the transmembrane pH gradient, were measured in washed cells of Rhizobium japonicum CC705 grown in cultures (5% O2-95% N2) in the presence of 10 mM KNO3 and in bacteroids from Glycine max. The delta p and its components remained reasonably constant in cells as well as in bacteroids at various stages of growth. The effects of uncouplers and ATPase inhibitors on the delta p and its components were determined in both cultured cells and bacteroids. The data indicated that a respiration-driven H+ translocation is the source of the delta p in both cultured cells and bacteroids.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gober J. W., Kashket E. R. H+/ATP stoichiometry of cowpea Rhizobium sp. strain 32H1 cells grown under nitrogen-fixing and nitrogen-nonfixing conditions. J Bacteriol. 1984 Oct;160(1):216–221. doi: 10.1128/jb.160.1.216-221.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kashket E. R., Blanchard A. G., Metzger W. C. Proton motive force during growth of Streptococcus lactis cells. J Bacteriol. 1980 Jul;143(1):128–134. doi: 10.1128/jb.143.1.128-134.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kashket E. R. Proton motive force in growing Streptococcus lactis and Staphylococcus aureus cells under aerobic and anaerobic conditions. J Bacteriol. 1981 Apr;146(1):369–376. doi: 10.1128/jb.146.1.369-376.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kashket E. R., Wong P. T. The intracellular pH of Escherichia coli. Biochim Biophys Acta. 1969 Oct 14;193(1):212–214. doi: 10.1016/0005-2736(69)90074-1. [DOI] [PubMed] [Google Scholar]
- Kumar S., Nicholas D. J. Proton electrochemical gradients in washed cells of Nitrosomonas europaea and Nitrobacter agilis. J Bacteriol. 1983 Apr;154(1):65–71. doi: 10.1128/jb.154.1.65-71.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laane C., Krone W., Konings W. N., Haaker H., Veeger C. The involvement of the membrane potential in nitrogen fixation by bacteroids of Rhizobium leguminosarum. FEBS Lett. 1979 Jul 15;103(2):328–332. doi: 10.1016/0014-5793(79)81355-1. [DOI] [PubMed] [Google Scholar]
- Matin A., Wilson B., Zychlinsky E., Matin M. Proton motive force and the physiological basis of delta pH maintenance in thiobacillus acidophilus. J Bacteriol. 1982 May;150(2):582–591. doi: 10.1128/jb.150.2.582-591.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shioi J. I., Matsuura S., Imae Y. Quantitative measurements of proton motive force and motility in Bacillus subtilis. J Bacteriol. 1980 Dec;144(3):891–897. doi: 10.1128/jb.144.3.891-897.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zablotowicz R. M., Eskew D. L., Focht D. D. Denitrification in Rhizobium. Can J Microbiol. 1978 Jun;24(6):757–760. doi: 10.1139/m78-126. [DOI] [PubMed] [Google Scholar]