Abstract
The total active transport of chloride ions across the gastric mucosa can be considered as the sum of two fractions; an acidic one which is equivalent to the acid secreted, and an electromotive one which accounts for the electric energy generated by the gastric mucosa. In the present studies, the relationship between this electromotive chloride transport and acid secretion has been investigated, using specific inhibitors. The rate of electromotive chloride transport was found to be essentially unaffected by changes in the rate of acid secretion, and also by inhibition of acid secretion by thiocyanate. On the other hand, diamox, in combination with histamine, was shown to depress or abolish the gastric electromotive force and to inhibit partially the total chloride transport, while acid was secreted at an almost normal rate. This kind of inhibition is undefined as to its mechanism but seems to be more specific for the gastric chloride transport than any other inhibitor known. It is concluded that acid secretion and electromotive chloride transport involve two different mechanisms, and are not absolutely essential for each other. The present results do not support the view that carbonic anhydrase is essential for acid secretion. They rather suggest an important function of this enzyme in the mechanism of active chloride transport.
Full Text
The Full Text of this article is available as a PDF (713.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Crane E. E., Davies R. E., Longmuir N. M. Relations between hydrochloric acid secretion and electrical phenomena in frog gastric mucosa. Biochem J. 1948;43(3):321–336. doi: 10.1042/bj0430321. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HEINZ E., DURBIN R. P. Studies of the chloride transport in the gastric mucosa of the frog. J Gen Physiol. 1957 Sep 20;41(1):101–117. doi: 10.1085/jgp.41.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOGBEN C. A. M. The chloride transport system of the gastric mucosa. Proc Natl Acad Sci U S A. 1951 Jul;37(7):393–395. doi: 10.1073/pnas.37.7.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOGBEN C. A. Active transport of chloride by isolated frog gastric epithelium; origin of the gastric mucosal potential. Am J Physiol. 1955 Mar;180(3):641–649. [PubMed] [Google Scholar]
- REHM W. S. Electrical resistance of resting and secreting stomach. Am J Physiol. 1953 Mar;172(3):689–699. doi: 10.1152/ajplegacy.1953.172.3.689. [DOI] [PubMed] [Google Scholar]
- SCHLESINGER H., DENNIS W. H., REHM W. S. Effect of thiocyanate on electrophysiological properties of mammalian stomach. Am J Physiol. 1955 Oct;183(1):75–78. doi: 10.1152/ajplegacy.1955.183.1.75. [DOI] [PubMed] [Google Scholar]
- TEORELL T. The acid-base balance of the secreting isolated gastric mucosa. J Physiol. 1951 Jul;114(3):267–276. doi: 10.1113/jphysiol.1951.sp004618. [DOI] [PMC free article] [PubMed] [Google Scholar]
- USSING H. H. Transport of ions across cellular membranes. Physiol Rev. 1949 Apr;29(2):127–155. doi: 10.1152/physrev.1949.29.2.127. [DOI] [PubMed] [Google Scholar]