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. 1958 Mar 20;41(4):767–782. doi: 10.1085/jgp.41.4.767

DISPOSITION OF INTRAPERITONEALLY INJECTED CALCIUM-45 IN SUCKLING RATS

Felix Bronner 1
PMCID: PMC2194878  PMID: 13514010

Abstract

The time course of the concentration of radiocalcium was studied in the serum, skeleton, pelt, muscles, and pooled internal organs of 10-day-old rats. Within 10 hours of injection, the specific activity of the tissue groups exceeded the specific activity of the serum and remained above it during the period studied (120 hours). Chemical and autoradiographic analyses showed how rapidly most of the injected Ca45 found its way into the skeleton. A model was constructed with the assumption that the skeleton constitutes an essentially irreversible reservoir for the tracer in a multicompartment system in which the blood is the central or feeding compartment. The rate of transfer of the tracer from the soft tissue compartments to the serum was calculated from the equation See PDF for Equation in which C = concentration in serum (expressed as a series of exponential terms) C' = concentration in a given soft tissue Substitution in the integrated form of this equation yielded equations which had the major properties of the empirical equations fitted to the experimental points. The relative order of transfer constants (k' –1) was: organs ≥ pelt > muscle.

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Selected References

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  1. ARNOLD J. S., JEE W. S., JOHNSON K. Observations and quantitative radioautographic studies of calcium45 deposited in vivo in forming Haversian systems and old bone of rabbit. Am J Anat. 1956 Sep;99(2):291–313. doi: 10.1002/aja.1000990205. [DOI] [PubMed] [Google Scholar]
  2. BOGOROCH J. G. R., NADLER N. J., LEBLOND C. P. The theory and methods of the radioautographic localization of radioelements in tissues. Am J Roentgenol Radium Ther. 1951 Mar;65(3):420–458. [PubMed] [Google Scholar]
  3. BRONNER F., HARRIS R. S., MALETSKOS C. J., BENDA C. E. Studies in calcium metabolism: effect of food phytates on calcium 45 uptake in children on low-calcium breakfasts. J Nutr. 1954 Dec 10;54(4):523–542. doi: 10.1093/jn/54.4.523. [DOI] [PubMed] [Google Scholar]
  4. BRONNER F., HARRIS R. S., MALETSKOS C. J., BENDA C. E. Studies in calcium metabolism; the fate of intravenously injected radiocalcium in human beings. J Clin Invest. 1956 Jan;35(1):78–88. doi: 10.1172/JCI103254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAHL L. K. A simple and sensitive histochemical method for calcium. Proc Soc Exp Biol Med. 1952 Jul;80(3):474–479. doi: 10.3181/00379727-80-19661. [DOI] [PubMed] [Google Scholar]
  6. DZIEWIATKOWSKI D. D. Radioautographic visualization of sulfur-35 disposition in the articular cartilage and bone of suckling rats following injection of labeled sodium sulfate. J Exp Med. 1951 May;93(5):451–458. doi: 10.1084/jem.93.5.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HANSARD S. L., COMAR C. L., DAVIS G. K. Effects of age upon the physiological behavior of calcium in cattle. Am J Physiol. 1954 Jun;177(3):383–389. doi: 10.1152/ajplegacy.1954.177.3.383. [DOI] [PubMed] [Google Scholar]
  8. LEBLOND C. P., WILKINSON G. W., BELANGER L. F., ROBICHON J. Radio-autographic visualization of bone formation in the rat. Am J Anat. 1950 Mar;86(2):289–341. doi: 10.1002/aja.1000860205. [DOI] [PubMed] [Google Scholar]
  9. PLUMLEE M. P., HANSARD S. L., COMAR C. L., BEESON W. M. Placental transfer and deposition of labeled calcium in the developing bovine fetus. Am J Physiol. 1952 Dec;171(3):678–686. doi: 10.1152/ajplegacy.1952.171.3.678. [DOI] [PubMed] [Google Scholar]
  10. SOLOMON A. K. Equations for tracer experiments. J Clin Invest. 1949 Nov;28(6 Pt 1):1297–1307. doi: 10.1172/JCI102197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. THOMAS R. O., LITOVITZ T. A., GESCHICKTER C. F. Alterations in dynamics of calcium metabolism by intraintestinal calcium reservoirs. Am J Physiol. 1954 Mar;176(3):381–387. doi: 10.1152/ajplegacy.1954.176.3.381. [DOI] [PubMed] [Google Scholar]
  12. THOMAS R. O., LITOVITZ T. A., RUBIN M. I., GESCHICKTER C. F. Dynamics of calcium metabolism; time distribution of intravenously administered radiocalcium. Am J Physiol. 1952 Jun;169(3):568–575. doi: 10.1152/ajplegacy.1952.169.3.568. [DOI] [PubMed] [Google Scholar]

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