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. 1958 Sep 20;42(1):109–136. doi: 10.1085/jgp.42.1.109

STUDIES ON THE ORIGIN OF BACTERIAL VIRUSES

I. THE INCIDENCE OF PHAGE-PRODUCING CELLS IN VARIOUS B. MEGATHERIUM CULTURES. II. THE EFFECT OF ULTRAVIOLET LIGHT ON THE INCIDENCE OF PHAGE-PRODUCING CELLS AND OF TERRAMYCIN-RESISTANT CELLS. III. THE EFFECT OF HYDROGEN PEROXIDE ON THE INCIDENCE OF PHAGE-PRODUCING CELLS AND OF TERRAMYCIN-RESISTANT CELLS. IV. CALCULATION OF THE INCIDENCE OF PHAGE-PRODUCING CELLS

John H Northrop 1; With the Technical Assistance of Marie King1
PMCID: PMC2194896  PMID: 13575777

Abstract

I. The Incidence of Phage-Producing Cells in Various B. megatherium Cultures Analyses of small samples containing a few cells each show that lysogenic B. megatherium produces phage particles in groups of from 10 to 1000 depending on the megatherium strain and the culture medium. These groups probably correspond to the number of particles produced by a single cell. The proportion of such phage-producing cells varies from <1 x 10-10 to about 1 x 10-2 depending on the megatherium strain and the culture medium. If a culture produces two types of phage, the different types usually appear in separate samples. If mixed samples occur, the number of such samples is about what would be expected for the probability that two separate groups would appear in one sample. This result indicates that the appearance of a distinct phage type is the result of a change in the bacterial cell rather than a change in a phage particle, since in the latter case a mixture of the two types would result. II. The Effect of Ultraviolet Light on the Incidence of Phage-Producing and of Terramycin-Resistant Cells in Various B. megatherium Cultures Low intensity of ultraviolet light increases the proportion of both phage-producing cells and of terramycin-resistant mutants. The increase in phage-producing cells is greater than the increase in terramycin-resistant cells. High intensities of ultraviolet light cause practically all the cells of some B. megatherium strains to produce phage. The number of terramycin-resistant mutants cannot be determined under these conditions. The effect of ultraviolet light varies, depending on the megatherium strain and the culture medium. III. The Effect of Hydrogen Peroxide on the Incidence of Phage-Producing and of Terramycin-Resistant Cells in Various B. megatherium Cultures Low concentrations of hydrogen peroxide increase the number of phage-producing cells and of terramycin-resistant cells, concomitantly, from two to five times. High concentrations of hydrogen peroxide cause almost all the cells of some strains of megatherium to produce phage. IV. Calculation of the Incidence of Phage-Producing Cells The time rate of the appearance of phage particles in normal cultures, or in cultures treated with ultraviolet light or hydrogen peroxide, may be calculated by the same equations which predict the occurrence of terramycin-resistant mutants in B. megatherium cultures. These equations predict that the number of mutants will increase more or less in proportion to the concentration of mutagenic agent, so long as the mutation rate remains very small compared to the growth rate. As the mutation rate approaches the growth rate, there will be a very rapid increase in the proportion of mutants. This explains the striking effect of higher concentrations of mutagenic agents. In order to calculate the results after exposure to strong ultraviolet light or hydrogen peroxide, it is necessary to assume that the change from normal to phage-producing cell occurs without cell division.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS M. H., PARK B. H. An enzyme produced by a phage-host cell system. II. The properties of the polysaccharide depolymerase. Virology. 1956 Dec;2(6):719–736. doi: 10.1016/0042-6822(56)90054-x. [DOI] [PubMed] [Google Scholar]
  2. BARON L. S., FORMAL S. B., SPILMAN W. Vi phage-host interaction in Salmonella typhosa. J Bacteriol. 1955 Feb;69(2):177–183. doi: 10.1128/jb.69.2.177-183.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen S. S., Anderson T. F. CHEMICAL STUDIES ON HOST-VIRUS INTERACTIONS : I. THE EFFECT OF BACTERIOPHAGE ADSORPTION ON THE MULTIPLICATION OF ITS HOST, ESCHERICHIA COLI B WITH AN APPENDIX GIVING SOME DATA ON THE COMPOSITION OF THE BACTERIOPHAGE, T2. J Exp Med. 1946 Oct 31;84(5):511–523. doi: 10.1084/jem.84.5.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FREDERICQ P. Localisation du prophage sur le chromosome; son intervention dans le taux de ségrégation apparent des rocombinants d'Escherichia coli K. 12. C R Seances Soc Biol Fil. 1954 Aug-Sep;148(15-18):1501–1504. [PubMed] [Google Scholar]
  5. FREDERICQ P. Recherches sur les facteurs génétiques déterminant les propriétés lysogènes et colicinogènes des E. coli. Rev Med Liege. 1954 Mar 1;9(5):150–156. [PubMed] [Google Scholar]
  6. Haas F. L., Doudney C. O. A RELATION OF NUCLEIC ACID SYNTHESIS TO RADIATION-INDUCED MUTATION FREQUENCY IN BACTERIA. Proc Natl Acad Sci U S A. 1957 Oct 15;43(10):871–883. doi: 10.1073/pnas.43.10.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JACOB F. Développement spontané et induit des bactériophages chez des Pseudomonas pyocyanea polylysogènes. Ann Inst Pasteur (Paris) 1952 Nov;83(5):671–692. [PubMed] [Google Scholar]
  8. Krueger A. P., Mundell J. H. THE DEMONSTRATION OF PHAGE PRECURSOR IN THE BACTERIAL CELL. Science. 1938 Dec 9;88(2293):550–551. doi: 10.1126/science.88.2293.550. [DOI] [PubMed] [Google Scholar]
  9. LEDERBERG J., EDWARDS P. R. Sero-typic recombination in Salmonella. J Immunol. 1953 Oct;71(4):232–240. [PubMed] [Google Scholar]
  10. LEDERBERG J. Viruses, genes, and cells. Bacteriol Rev. 1957 Sep;21(3):133–139. doi: 10.1128/br.21.3.133-139.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LWOFF A. Conditions de l'efficacité inductrice du rayonnement ultra-violet chez une bactérie lysogène. Ann Inst Pasteur (Paris) 1951 Oct;81(4):370–388. [PubMed] [Google Scholar]
  12. LWOFF A., SIMINOVITCH L., KJELDGAARD N. Induction de la production de bacteriophages chez une bactérie lysogène. Ann Inst Pasteur (Paris) 1950 Dec;79(6):815–859. [PubMed] [Google Scholar]
  13. LWOFF A. The concept of virus. J Gen Microbiol. 1957 Oct;17(2):239–253. doi: 10.1099/00221287-17-2-239. [DOI] [PubMed] [Google Scholar]
  14. MILLER E. M., GOEBEL W. F. The nature of prophage in lysogenic Bacillus megatherium. J Exp Med. 1954 Dec 1;100(6):525–540. doi: 10.1084/jem.100.6.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. NORTHROP J. H. Adaptation of Bacillus megatherium to terramycin (oxytetracycline). J Gen Physiol. 1957 Mar 20;40(4):547–563. doi: 10.1085/jgp.40.4.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. NORTHROP J. H. Apparatus for maintaining bacterial cultures in the steady state. J Gen Physiol. 1954 Sep 20;38(1):105–115. doi: 10.1085/jgp.38.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. NORTHROP J. H. Growth and phage production of B. megatherium. I. Growth of cells after infection with C phage. II. Rate of growth, phage yield, and RNA content of cells. III. Effect of various substances on growth rate and phage production. J Gen Physiol. 1953 Mar;36(4):581–599. doi: 10.1085/jgp.36.4.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NORTHROP J. H. Growth and phage production of lysogenic B. megatherium. J Gen Physiol. 1951 May;34(5):715–735. doi: 10.1085/jgp.34.5.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. NORTHROP J. H., MURPHY J. S. Appearance of new phage types and new lysogenic strains after adaptation of lysogenic B. megatherium to ammonium sulfate culture medium. J Gen Physiol. 1956 Mar 20;39(4):607–624. doi: 10.1085/jgp.39.4.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. NORTHROP J. H. The effect of ultraviolet and white light on growth rate, lysis, and phage production of Bacillus megatherium. J Gen Physiol. 1957 May 20;40(5):653–661. doi: 10.1085/jgp.40.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. NORTHROP J. H. The proportion of terramycin-resistant mutants in B. megatherium cultures. J Gen Physiol. 1957 Sep 20;41(1):131–141. doi: 10.1085/jgp.41.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. RAETTIG H. Experimentelle Untersuchungen über Entwicklungsvorgänge bei Bakteriophagen. VI. Der Lysintiter als Funktion der Bebrütungstemperatur des lysintragenden Bakterienstammes. Zentralbl Bakteriol Orig. 1955 Jun;163(2-3):132–148. [PubMed] [Google Scholar]
  23. STOCKER B. A. Transduction of flagellar characters in Salmonella. J Gen Microbiol. 1953 Dec;9(3):410–433. doi: 10.1099/00221287-9-3-410. [DOI] [PubMed] [Google Scholar]

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