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. 1959 Mar 20;42(4):803–815. doi: 10.1085/jgp.42.4.803

CALCIUM INFLUX IN SKELETAL MUSCLE AT REST, DURING ACTIVITY, AND DURING POTASSIUM CONTRACTURE

C Paul Bianchi 1, A M Shanes 1
PMCID: PMC2195004  PMID: 13631205

Abstract

Calcium influx in the sartorius muscle of the frog (Rana pipiens) has been estimated from the rate of entry of Ca45. In the unstimulated preparation it is about equal to what has been reported for squid giant axons, but that per impulse is at least 30 times greater than in nerve fibers. The enhanced twitch when NO- 2 replaces Cl- in Ringer's is associated with at least a 60 per cent increase in influx during activity, whereas this anion substitution does not affect the passive influx significantly. Calcium entry during potassium contracture is even more markedly augmented than during electrical stimulation, but only at the beginning of the contracture; thus, when a brief Ca45 exposure precedes excess K+ application, C45 uptake is increased three- to fivefold over the controls not subjected to K+, whereas when C45 and K+ are added together, no measurable increase in Ca45 uptake occurs. These findings are in keeping with the brevity of potassium contracture in "fast (twitch)" fibers such as in sartorius muscle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENOIT P. H., CORABOEUF E. Modifications électrotoniques du potentiel de pointe et du potentiel consécutif de la fibre musculaire striée. C R Seances Soc Biol Fil. 1955 Jul;149(13-14):1435–1438. [PubMed] [Google Scholar]
  2. FENN W. O., GILBERT D. L. Calcium equilibrium in muscle. J Gen Physiol. 1957 Jan 20;40(3):393–408. doi: 10.1085/jgp.40.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FRANK G. B. Negative after-potential of frog's skeletal muscle. J Neurophysiol. 1957 Nov;20(6):602–614. doi: 10.1152/jn.1957.20.6.602. [DOI] [PubMed] [Google Scholar]
  4. HODGKIN A. L., KEYNES R. D. Movements of labelled calcium in squid giant axons. J Physiol. 1957 Sep 30;138(2):253–281. doi: 10.1113/jphysiol.1957.sp005850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HUXLEY A. F., NIEDERGERKE R. Structural changes in muscle during contraction; interference microscopy of living muscle fibres. Nature. 1954 May 22;173(4412):971–973. doi: 10.1038/173971a0. [DOI] [PubMed] [Google Scholar]
  6. HUXLEY A. F., TAYLOR R. E. Function of Krause's membrane. Nature. 1955 Dec 3;176(4492):1068–1068. doi: 10.1038/1761068a0. [DOI] [PubMed] [Google Scholar]
  7. HUXLEY H., HANSON J. Changes in the cross-striations of muscle during contraction and stretch and their structural interpretation. Nature. 1954 May 22;173(4412):973–976. doi: 10.1038/173973a0. [DOI] [PubMed] [Google Scholar]
  8. KAHN A. J., SANDOW A. The potentiation of muscular contraction by the nitrate-ion. Science. 1950 Dec 1;112(2918):647–649. doi: 10.1126/science.112.2918.647. [DOI] [PubMed] [Google Scholar]
  9. LUBIN M. The effect of iodide and thiocyanate ions on the mechanical and electrical properties of frog muscle. J Cell Physiol. 1957 Apr;49(2):335–349. doi: 10.1002/jcp.1030490214. [DOI] [PubMed] [Google Scholar]
  10. NIEDERGERKE R. The potassium chloride contracture of the heart and its modification by calcium. J Physiol. 1956 Dec 28;134(3):584–599. doi: 10.1113/jphysiol.1956.sp005667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PADSHA S. M. The influence of anions on the membrane resistance of skeletal muscle. J Physiol. 1957 Jun 18;137(1):26–8P. [PubMed] [Google Scholar]
  12. RITCHIE J. M. The effect of nitrate on the active state of muscle. J Physiol. 1954 Oct 28;126(1):155–168. doi: 10.1113/jphysiol.1954.sp005200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SHANES A. M. Drug and ion effects in frog muscle. J Gen Physiol. 1950 Jul 20;33(6):729–744. doi: 10.1085/jgp.33.6.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SHANES A. M. Electrochemical aspects of physiological and pharmacological action in excitable cells. I. The resting cell and its alteration by extrinsic factors. Pharmacol Rev. 1958 Mar;10(1):59–164. [PubMed] [Google Scholar]
  15. SHANES A. M. Electrochemical aspects of physiological and pharmacological action in excitable cells. II. The action potential and excitation. Pharmacol Rev. 1958 Jun;10(2):165–273. [PubMed] [Google Scholar]
  16. SHANES A. M. Potassium movement in relation to nerve activity. J Gen Physiol. 1951 Jul;34(6):795–807. doi: 10.1085/jgp.34.6.795. [DOI] [PMC free article] [PubMed] [Google Scholar]

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