Abstract
The electrokinetic stability of washed normal human erythrocytes is discussed from the point of view of pH, ionic strength, and composition of the suspending medium. Many of the electrophoretic characteristics at low ionic strengths (sorbitol to maintain the tonicity), such as the isopotential points, are shown to arise principally from adsorption of hemolysate. The concept of electrokinetically stable, metastable, and unstable states for the red cell at various ionic strengths is introduced in preference to the general term "cell injury." In the stable state which exists around pH 7.4 for ionic strengths >0.007, no adsorption of hemolysate occurs, in the metastable state reversible adsorption of hemolysate occurs, and in the unstable state, in which ionic strengths and pH ranges are outside the metastable range, the membrane undergoes irreversible hemolysate adsorption or more general hydrolytic degradation. It is deduced from the equivalent binding of CNS, I, Cl, and F, the pH mobility relationships, and the conformation of the ionic strength data in the stable state to a Langmuir adsorption isotherm, that the membrane of the human erythrocyte behaves as a macropolyanion whose properties are modified by gegen ion association and in some instances by hemolysate adsorption. The experimental results are insufficient to establish conclusively the nature of the ionogenic groupings present in the membrane interphase.
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Selected References
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- BANGHAM A. D., HEARD D. H., FLEMANS R., SEAMAN G. V. An apparatus for microelectrophoresis of small particles. Nature. 1958 Sep 6;182(4636):642–644. doi: 10.1038/182642a0. [DOI] [PubMed] [Google Scholar]
- BANGHAM A. D., PETHICA B. A., SEAMAN G. V. The charged groups at the interface of some blood cells. Biochem J. 1958 May;69(1):12–19. doi: 10.1042/bj0690012. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BATEMAN J. B., ZELLNER A. The electrophoretic properties of red blood cells: the effect of changing pH and ionic strength. Arch Biochem Biophys. 1956 Jan;60(1):44–51. doi: 10.1016/0003-9861(56)90394-0. [DOI] [PubMed] [Google Scholar]
- KLOTZ I. M. The nature of some ion-protein complexes. Cold Spring Harb Symp Quant Biol. 1950;14:97–112. doi: 10.1101/sqb.1950.014.01.014. [DOI] [PubMed] [Google Scholar]
- LONDON I. M., SCHWARZ H. Erythrocyte metabolism; the metabolic behavior of the cholesterol of human erythrocytes. J Clin Invest. 1953 Dec;32(12):1248–1252. doi: 10.1172/JCI102853. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOVELOCK J. E. The physical instability of human red blood cells. Biochem J. 1955 Aug;60(4):692–696. doi: 10.1042/bj0600692. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PETHICA B. A. Lysis by physical and chemical methods. J Gen Microbiol. 1958 Apr;18(2):473–480. doi: 10.1099/00221287-18-2-473. [DOI] [PubMed] [Google Scholar]
- Stearns T. W., Roepke M. H. Electrophoresis Studies on Brucella. J Bacteriol. 1941 Sep;42(3):411–430. doi: 10.1128/jb.42.3.411-430.1941. [DOI] [PMC free article] [PubMed] [Google Scholar]