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. 1961 Mar 1;44(4):689–712. doi: 10.1085/jgp.44.4.689

Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells

Guillermo Whittembury 1, Nobuhiro Sugino 1, A K Solomon 1
PMCID: PMC2195119  PMID: 13784943

Abstract

The cellular concentrations of Na, K, and Cl have been measured in kidney slices of the amphibian, Necturus maculosus. Permeability coefficients have been determined for Na, K, Cl, Rb, Cs, and choline, from studies both of the uptake of radioactive isotopes and the rate of cell swelling in anisotonic solutions. The results of both methods were found to agree well. Measurements were also made of electrical potential differences across the peritubular face of the kidney cells using bathing solutions in which the electrolyte composition and concentrations could be varied. The data obtained are consistent with a model cell in which the potential difference arises as a result of differences in Na permeability relative to K on the two faces of the cell. The intracellular Na concentration is considered to be regulated by a Na-K coupled pump located at the peritubular face of the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOTT P. A. Renal excretion of creatinine in Necturus; a reinvestigation by direct analysis of glomerular and tubule fluid for creatinine and inulin. Am J Physiol. 1952 Jan;168(1):107–113. doi: 10.1152/ajplegacy.1951.168.1.107. [DOI] [PubMed] [Google Scholar]
  2. BOTT P. A. The concentration of potassium in glomerular urine of necturi. J Biol Chem. 1955 Jul;215(1):287–293. [PubMed] [Google Scholar]
  3. Boyle P. J., Conway E. J. Potassium accumulation in muscle and associated changes. J Physiol. 1941 Aug 11;100(1):1–63. doi: 10.1113/jphysiol.1941.sp003922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CAREY M. J., CONWAY E. J. Comparison of various media for immersing frog sartorii at room temperature, and evidence for the regional distribution of fibre Na+. J Physiol. 1954 Aug 27;125(2):232–250. doi: 10.1113/jphysiol.1954.sp005154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DURBIN R. P. Osmotic flow of water across permeable cellulose membranes. J Gen Physiol. 1960 Nov;44:315–326. doi: 10.1085/jgp.44.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GIEBISCH G. Measurements of electrical potentials and ion fluxes on single renal tubules. Circulation. 1960 May;21:879–891. doi: 10.1161/01.cir.21.5.879. [DOI] [PubMed] [Google Scholar]
  7. GIEBISCH G. Measurements of pH, chloride and insulin concentrations in proximal tubule fluid of necturus. Am J Physiol. 1956 Apr;185(1):171–174. doi: 10.1152/ajplegacy.1956.185.1.171. [DOI] [PubMed] [Google Scholar]
  8. GOLDSTEIN D. A., SOLOMON A. K. Determination of equivalent pore radius for human red cells by osmotic pressure measurement. J Gen Physiol. 1960 Sep;44:1–17. doi: 10.1085/jgp.44.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KOEFOED-JOHNSEN V., USSING H. H. The nature of the frog skin potential. Acta Physiol Scand. 1958 Jun 2;42(3-4):298–308. doi: 10.1111/j.1748-1716.1958.tb01563.x. [DOI] [PubMed] [Google Scholar]
  10. MULLINS Lj. The penetration of some cations into muscle. J Gen Physiol. 1959 Mar 20;42(4):817–829. doi: 10.1085/jgp.42.4.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RENKIN E. M. Filtration, diffusion, and molecular sieving through porous cellulose membranes. J Gen Physiol. 1954 Nov 20;38(2):225–243. [PMC free article] [PubMed] [Google Scholar]
  12. SHIPP J. C., HANENSON I. B., WINDHAGER E. E., SCHATZMANN H. J., WHITTEMBURY G., YOSHIMURA H., SOLOMON A. K. Single proximal tubules of the Necturus kidney; methods for micropuncture and microperfusion. Am J Physiol. 1958 Dec;195(3):563–569. doi: 10.1152/ajplegacy.1958.195.3.563. [DOI] [PubMed] [Google Scholar]
  13. WHITTAM R. The permeability of kidney cortex to chloride. J Physiol. 1956 Mar 28;131(3):542–554. doi: 10.1113/jphysiol.1956.sp005481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WHITTEMBURY G., RAMIREZ M., FERNANDEZ J., MONGE C. The use of high resistance voltmeters in the determination of biological chlorides. Acta Physiol Lat Am. 1957;7(2):76–85. [PubMed] [Google Scholar]
  15. WHITTEMBURY G., SUGINO N., SOLOMON A. K. Effect of antidiuretic hormone and calcium on the equivalent pore radius of kidney slices from Necturus. Nature. 1960 Aug 20;187:699–701. doi: 10.1038/187699a0. [DOI] [PubMed] [Google Scholar]
  16. WHITTEMBURY G., WINDHAGER E. E. Electrical potential difference measurements in perfused single proximal tubules of Necturus kidney. J Gen Physiol. 1961 Mar;44:679–687. doi: 10.1085/jgp.44.4.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WINDHAGER E. E., WHITTEMBURY G., OKEN D. E., SCHATZMANN H. J., SOLOMON A. K. Single proximal tubules of the Necturus kidney. III. Dependence of H2O movement on NaCl concentration. Am J Physiol. 1959 Aug;197:313–318. doi: 10.1152/ajplegacy.1959.197.2.313. [DOI] [PubMed] [Google Scholar]

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