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. 1961 Mar 1;44(4):713–742. doi: 10.1085/jgp.44.4.713

Magnesium and Calcium in Isolated Cell Nuclei

H Naora 1, H Naora 1, A E Mirsky 1, V G Allfrey 1
PMCID: PMC2195122  PMID: 13727745

Abstract

The calcium and magnesium contents of thymus nuclei have been determined and the nuclear sites of attachment of these two elements have been studied. The nuclei used for these purposes were isolated in non-aqueous media and in sucrose solutions. Non-aqueous nuclei contain 0.024 per cent calcium and 0.115 per cent magnesium. Calcium and magnesium are held at different sites. The greater part of the magnesium is bound to DNA, probably to its phosphate groups. Evidence is presented that the magnesium atoms combined with the phosphate groups of DNA are also attached to mononucleotides. There is reason to believe that those DNA-phosphate groups to which magnesium is bound, less than 1/10th of the total, are metabolically active, while those to which histones are attached seem to be inactive.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLFREY V. G., MIRSKY A. E., OSAWA S. Mononucleotides of the cell nucleus. J Gen Physiol. 1957 Jan 20;40(3):491–513. [PMC free article] [PubMed] [Google Scholar]
  2. ALLFREY V. G., MIRSKY A. E., OSAWA S. Protein synthesis in isolated cell nuclei. J Gen Physiol. 1957 Jan 20;40(3):451–490. doi: 10.1085/jgp.40.3.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ALLFREY V., STERN H., MIRSKY A. E., SAETREN H. The isolation of cell nuclei in non-aqueous media. J Gen Physiol. 1952 Jan;35(3):529–554. doi: 10.1085/jgp.35.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Allfrey V. G., Mirsky A. E. SOME ASPECTS OF RIBONUCLEIC ACID SYNTHESIS IN ISOLATED CELL NUCLEI. Proc Natl Acad Sci U S A. 1957 Sep 15;43(9):821–826. doi: 10.1073/pnas.43.9.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Allfrey V. G., Mirsky A. E. SOME EFFECTS OF SUBSTITUTING THE DEOXYRIBONUCLEIC ACID OF ISOLATED NUCLEI WITH OTHER POLYELECTROLYTES. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):981–991. doi: 10.1073/pnas.44.10.981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CHAO F. C. Dissociation of macromolecular ribonucleoprotein of yeast. Arch Biochem Biophys. 1957 Aug;70(2):426–431. doi: 10.1016/0003-9861(57)90130-3. [DOI] [PubMed] [Google Scholar]
  7. DALY M. M., ALLFREY V. G., MIRSKY A. E. Purine and pyrimidine contents of some desoxypentose nucleic acids. J Gen Physiol. 1950 May 20;33(5):497–510. doi: 10.1085/jgp.33.5.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DALY M. M., MIRSKY A. E. Histones with high lysine content. J Gen Physiol. 1955 Jan 20;38(3):405–413. doi: 10.1085/jgp.38.3.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FELSENFELD G., HUANG S. The interaction of polynucleotides with cations. Biochim Biophys Acta. 1959 Jul;34:234–242. doi: 10.1016/0006-3002(59)90253-7. [DOI] [PubMed] [Google Scholar]
  10. GRUNBERG-MANAGO M., ORTIZ P. J., OCHOA S. Enzymic synthesis of polynucleotides. I. Polynucleotide phosphorylase of azotobacter vinelandii. Biochim Biophys Acta. 1956 Apr;20(1):269–285. doi: 10.1016/0006-3002(56)90286-4. [DOI] [PubMed] [Google Scholar]
  11. HOAGLAND M. B., KELLER E. B., ZAMECNIK P. C. Enzymatic carboxyl activation of amino acids. J Biol Chem. 1956 Jan;218(1):345–358. [PubMed] [Google Scholar]
  12. KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. Harvey Lect. 1957;53:83–112. [PubMed] [Google Scholar]
  13. KUNITZ M. Crystalline desoxyribonuclease; digestion of thymus nucleic acid; the kinetics of the reaction. J Gen Physiol. 1950 Mar;33(4):363–377. doi: 10.1085/jgp.33.4.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Levine R. P. CHROMOSOME STRUCTURE AND THE MECHANISM OF CROSSING OVER. Proc Natl Acad Sci U S A. 1955 Oct 15;41(10):727–730. doi: 10.1073/pnas.41.10.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mazia D. THE PARTICULATE ORGANIZATION OF THE CHROMOSOME. Proc Natl Acad Sci U S A. 1954 Jun;40(6):521–527. doi: 10.1073/pnas.40.6.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. ORANGE M., RHEIN H. C. Microestimation of magnesium in body fluids. J Biol Chem. 1951 Mar;189(1):379–386. [PubMed] [Google Scholar]
  17. SCHNEIDER R. M. The effect of anions on the optical properties of rat liver nuclei isolated in glycerol solutions. Exp Cell Res. 1955 Feb;8(1):24–34. doi: 10.1016/0014-4827(55)90040-9. [DOI] [PubMed] [Google Scholar]
  18. SHACK J., BYNUM B. S. Determination of the interaction of deoxyribonucleate and magnesium ions by means of a metal ion indicator. Nature. 1959 Aug 22;184(Suppl 9):635–636. doi: 10.1038/184635a0. [DOI] [PubMed] [Google Scholar]
  19. SINSHEIMER R. L. The action of pancreatic desoxyribonuclease. I. Isolation of mono- and dinucleotides. J Biol Chem. 1954 May;208(1):445–459. [PubMed] [Google Scholar]
  20. STAEHELIN M. Effect of magnesium ions on the reactivity of ribonucleic acid. Experientia. 1959 Nov 15;15:413–414. doi: 10.1007/BF02157682. [DOI] [PubMed] [Google Scholar]
  21. STEFFENSEN D., BERGERON J. A. Autoradiographs of pollen tube nuclei with calcium-45. J Biophys Biochem Cytol. 1959 Dec;6:339–342. doi: 10.1083/jcb.6.3.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. THIERS R. E., VALLEE B. L. Distribution of metals in subcellular fractions of rat liver. J Biol Chem. 1957 Jun;226(2):911–920. [PubMed] [Google Scholar]
  23. TS'O P. O., BONNER J., VINOGRAD J. Structure and properties of microsomal nucleoprotein particles from pea seedlings. Biochim Biophys Acta. 1958 Dec;30(3):570–582. doi: 10.1016/0006-3002(58)90104-5. [DOI] [PubMed] [Google Scholar]
  24. WEBB J. M. A sensitive method for the determination of ribonucleic acid in tissues and microorganisms. J Biol Chem. 1956 Aug;221(2):635–649. [PubMed] [Google Scholar]
  25. WEISSMAN N., FISHER J. On the significance of magnesium ion for desoxyribonuclease activity. J Biol Chem. 1949 Apr;178(2):1007–1007. [PubMed] [Google Scholar]

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