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. 1962 Sep 1;46(1):131–142. doi: 10.1085/jgp.46.1.131

Water Loss during Contracture of Muscle

Benjamin Kaminer 1
PMCID: PMC2195250  PMID: 14453453

Abstract

The relationship of contracture and exudation of water in frozenthawed frog muscle was studied. With maximum shortening, there was a water loss of 35 per cent of the weight of muscle. By restricting the contraction, it was demonstrated that the amount of water loss was proportional to the degree of shortening, there being no significant loss with isometric contraction. Muscle already shortened by tetanic stimulation also exuded water on subsequent freezing and thawing. The force of contraction could be reduced by depleting the muscle of calcium and it was shown that the amount of water exuded was also proportional to the tensile ability of the muscle. In a smooth muscle (anterior byssus retractor of Mytilus) which did not contract vigorously only a little water exuded. Contracture produced by caffeine was similarly associated with a loss of water. Microscopic studies revealed a disruption of the sarcomeres of the frozen-thawed muscle which contracted; glycerol-extracted and calcium-depleted muscles, which did not contract on freeze-thawing, did not show such disruption. Freezing and thawing of actomyosin caused a reversible syneresis of the protein. It is concluded that the exudation of the water is not merely due to the freezing and thawing but is also dependent on the contractile events.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CARLSEN F., KNAPPEIS G. G., BUCHTHAL F. Ultrastructure of the resting and contracted striated muscle fiber at different degrees of stretch. J Biophys Biochem Cytol. 1961 Oct;11:95–117. doi: 10.1083/jcb.11.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CONWAY E. J. Nature and significance of concentration relations of potassium and sodium ions in skeletal muscle. Physiol Rev. 1957 Jan;37(1):84–132. doi: 10.1152/physrev.1957.37.1.84. [DOI] [PubMed] [Google Scholar]
  3. Conway D., Sakai T. CAFFEINE CONTRACTURE. Proc Natl Acad Sci U S A. 1960 Jun;46(6):897–903. doi: 10.1073/pnas.46.6.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HUXLEY A. F. Muscle structure and theories of contraction. Prog Biophys Biophys Chem. 1957;7:255–318. [PubMed] [Google Scholar]
  5. HUXLEY A. F., NIEDERGERKE R. Structural changes in muscle during contraction; interference microscopy of living muscle fibres. Nature. 1954 May 22;173(4412):971–973. doi: 10.1038/173971a0. [DOI] [PubMed] [Google Scholar]
  6. HUXLEY H., HANSON J. Changes in the cross-striations of muscle during contraction and stretch and their structural interpretation. Nature. 1954 May 22;173(4412):973–976. doi: 10.1038/173973a0. [DOI] [PubMed] [Google Scholar]
  7. JOHNSON W. H., KAHN J. S., SZENTGYORGYI A. G. Paramyosin and contraction of catch muscles. Science. 1959 Jul 17;130(3368):160–161. doi: 10.1126/science.130.3368.160. [DOI] [PubMed] [Google Scholar]
  8. KLOTZ I. M. Protein hydration and behavior; many aspects of protein behavior can be interpreted in terms of frozen water of hydration. Science. 1958 Oct 10;128(3328):815–822. doi: 10.1126/science.128.3328.815. [DOI] [PubMed] [Google Scholar]
  9. MARSH B. B., THOMPSON J. F. Thaw rigor and the delta state of muscle. Biochim Biophys Acta. 1957 May;24(2):427–428. doi: 10.1016/0006-3002(57)90216-0. [DOI] [PubMed] [Google Scholar]
  10. MARSH B. B. The effects of adenosine triphosphate on the fibre volume of a muscle homogenate. Biochim Biophys Acta. 1952 Sep;9(3):247–260. doi: 10.1016/0006-3002(52)90159-5. [DOI] [PubMed] [Google Scholar]
  11. ODEBLAD E. Studies on vaginal contents and cells with proton magnetic resonance. Ann N Y Acad Sci. 1959 Nov 18;83:189–206. doi: 10.1111/j.1749-6632.1960.tb40892.x. [DOI] [PubMed] [Google Scholar]
  12. PERRY S. V. Studies on the rigor resulting from the thawing of frozen frog sartorius muscle. J Gen Physiol. 1950 May 20;33(5):563–577. doi: 10.1085/jgp.33.5.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RAPATZ G., LUYET B. On the mechanism of ice formation and propagation in muscle. Biodynamica. 1959 Dec;8:121–144. [PubMed] [Google Scholar]
  14. SZENT-GYORGYI A. G. Effect of freezing and thawing on muscle. Enzymologia. 1950 Nov 15;14(4):252–253. [PubMed] [Google Scholar]
  15. SZENT-GYORGYI A. Free-energy relations and contraction of actomyosin. Biol Bull. 1949 Apr;96(2):140–161. [PubMed] [Google Scholar]
  16. SZENTKIRALYI E. M. Changes in the nucleotides of the cross-striated muscle after freezing. Arch Biochem Biophys. 1957 Apr;67(2):298–301. doi: 10.1016/0003-9861(57)90283-7. [DOI] [PubMed] [Google Scholar]
  17. WEBER A., WINICUR S. The role of calcium in the superprecipitation of actomyosin. J Biol Chem. 1961 Dec;236:3198–3202. [PubMed] [Google Scholar]

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