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. 1964 Jul 1;47(6):1061–1078. doi: 10.1085/jgp.47.6.1061

Turnover of Phosphatidic Acid and Sodium Extrusion from Mammalian Erythrocytes

Leonard B Kirschner 1, Jennifer Barker 1
PMCID: PMC2195374  PMID: 14192545

Abstract

Phosphatidic acid (PA) from swine and beef RBCs was isolated by chromatography on silicic acid columns. It comprised about 1 per cent of the total lipid phosphate in RBCs, but was eluted nearly pure from columns. An uncharacterized inositide accounted for 5 to 10 per cent of the phosphate in the PA-containing fraction. When cells were incubated with HP32O4 =, the fraction containing PA became more radioactive than any of the other fractions obtained. However, analysis of the labeled material by paper chromatography showed that most of the P32 was in the inositide, not in PA. With the assumption of kinetic homogeneity for cellular PA, compartmental analysis of the kinetics of tracer incorporation showed that PA turnover is 3 to 4 orders of magnitude too slow to account for sodium extrusion by these cells.

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Selected References

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  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. BORUT A., SCHMIDT-NIELSEN K. Respiration of avian salt-secreting gland in tissue slice experiments. Am J Physiol. 1963 Apr;204:573–581. doi: 10.1152/ajplegacy.1963.204.4.573. [DOI] [PubMed] [Google Scholar]
  3. BROCKERHOFF H., BALLOU C. E. Phosphate incorporation in brain phosphionositides. J Biol Chem. 1962 Jan;237:49–52. [PubMed] [Google Scholar]
  4. CURRAN P. F., LIONETTI F., SOLOMON A. K. Possible cation-carrier substances in blood. Nature. 1956 Sep 15;178(4533):582–583. doi: 10.1038/178582a0. [DOI] [PubMed] [Google Scholar]
  5. DAWSON R. M., HEMINGTON N., LINDSAY D. B. The phospholipids of the erythrocyte 'ghosts' of various species. Biochem J. 1960 Nov;77:226–230. doi: 10.1042/bj0770226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HAJDU S., LEONARD E. The cellular basis of cardiac glycoside action. Pharmacol Rev. 1959 Jun;11(2 Pt 1):173–209. [PubMed] [Google Scholar]
  7. HANAHAN D. J., DITTMER J. C., WARASHINA E. A column chromatographic separation of classes of phospholipides. J Biol Chem. 1957 Oct;228(2):685–700. [PubMed] [Google Scholar]
  8. HANAHAN D. J., TURNER M. B., JAYKO M. E. The isolation of egg phosphatidyl choline by an adsorption column technique. J Biol Chem. 1951 Oct;192(2):623–628. [PubMed] [Google Scholar]
  9. HAYASHI K., KANOH T., SHIMIZU S., KAI M., YAMAZOE S. Studies on the brain phospholipids. I. Incorporation of P32 into phospholipids in guinea-pig brain slices. J Biochem. 1962 Jan;51:63–71. doi: 10.1093/oxfordjournals.jbchem.a127501. [DOI] [PubMed] [Google Scholar]
  10. HOKIN L. E., HOKIN M. R. Acetylcholine and the exchange of phosphate in phosphatidic acid in brain microsomes. J Biol Chem. 1958 Oct;233(4):822–826. [PubMed] [Google Scholar]
  11. HOKIN L. E., HOKIN M. R. Diglyceride kinase and other path ways for phosphatidic acid synthesis in the erythrocyte membrane. Biochim Biophys Acta. 1963 Mar 12;67:470–484. doi: 10.1016/0006-3002(63)91852-3. [DOI] [PubMed] [Google Scholar]
  12. HOKIN L. E., HOKIN M. R. Studies on the carrier function of phosphatidic acid in sodium transport. I. The turnover of phosphatidic acid and phosphoinositide in the avian salt gland on stimulation of secretion. J Gen Physiol. 1960 Sep;44:61–85. doi: 10.1085/jgp.44.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HOKIN M. R., HOKIN L. E. The synthesis of phosphatidic acid from diglyceride and adenosine triphosphate in extracts of brain microsomes. J Biol Chem. 1959 Jun;234(6):1381–1386. [PubMed] [Google Scholar]
  14. KIRSCHNER L. B., HARDING N. The effect of adenosine on phosphate esters and sodium extrusion in swine erythrocytes. Arch Biochem Biophys. 1958 Sep;77(1):54–61. doi: 10.1016/0003-9861(58)90040-7. [DOI] [PubMed] [Google Scholar]
  15. KIRSCHNER L. B. The cation content of phospholipides from swine erythrocytes. J Gen Physiol. 1958 Nov 20;42(2):231–241. doi: 10.1085/jgp.42.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LASSEN U. V., THAYSEN J. H. Correlation between sodium transport and oxygen consumption in isolated renal tissue. Biochim Biophys Acta. 1961 Mar 4;47:616–618. doi: 10.1016/0006-3002(61)90567-4. [DOI] [PubMed] [Google Scholar]
  17. LEA C. H., RHODES D. N. The ninhydrin reaction of unhydrolysed phospholipids. Biochim Biophys Acta. 1955 Jul;17(3):416–423. doi: 10.1016/0006-3002(55)90391-7. [DOI] [PubMed] [Google Scholar]
  18. LEAF A., RENSHAW A. Ion transport and respiration of isolated frog skin. Biochem J. 1957 Jan;65(1):82–90. doi: 10.1042/bj0650082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MARINETTI G. V., STOTZ E. Chromatography of phosphatides on silicic acid impregnated paper. Biochim Biophys Acta. 1956 Jul;21(1):168–170. doi: 10.1016/0006-3002(56)90108-1. [DOI] [PubMed] [Google Scholar]
  20. NAGANO K., NAKAO M. Cation carrier in the erythrocyte membrane. J Biochem. 1962 Aug;52:99–102. doi: 10.1093/oxfordjournals.jbchem.a127588. [DOI] [PubMed] [Google Scholar]
  21. POST R. L., MERRITT C. R., KINSOLVING C. R., ALBRIGHT C. D. Membrane adenosine triphosphatase as a participant in the active transport of sodium and potassium in the human erythrocyte. J Biol Chem. 1960 Jun;235:1796–1802. [PubMed] [Google Scholar]
  22. PRANKERD T. A., ALTMAN K. I. A study of the metabolism of phosphorus in mammalian red cells. Biochem J. 1954 Dec;58(4):622–633. doi: 10.1042/bj0580622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. RAPPORT M. M., ALONZO N. Photometric determination of fatty acid ester groups in phospholipides. J Biol Chem. 1955 Nov;217(1):193–198. [PubMed] [Google Scholar]
  24. SCHATZMANN H. J. Herzglykoside als Hemmstoffe für den aktiven Kalium- und Natriumtransport durch die Erythrocytenmembran. Helv Physiol Pharmacol Acta. 1953;11(4):346–354. [PubMed] [Google Scholar]
  25. SKOU J. C. The influence of some cations on an adenosine triphosphatase from peripheral nerves. Biochim Biophys Acta. 1957 Feb;23(2):394–401. doi: 10.1016/0006-3002(57)90343-8. [DOI] [PubMed] [Google Scholar]
  26. SORENSON A. L., KIRSCHNER L. B., BARKER J. Sodium fluxes in the erythrocytes of swine, ox, and dog. J Gen Physiol. 1962 Jul;45:1031–1047. doi: 10.1085/jgp.45.6.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. TOSTESON D. C., HOFFMAN J. F. Regulation of cell volume by active cation transport in high and low potassium sheep red cells. J Gen Physiol. 1960 Sep;44:169–194. doi: 10.1085/jgp.44.1.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. WHITTAM R. Potassium movements and ATP in human red cells. J Physiol. 1958 Mar 11;140(3):479–497. [PMC free article] [PubMed] [Google Scholar]
  29. WILBRANDT W. Transport through biological membranes. Annu Rev Physiol. 1963;25:601–630. doi: 10.1146/annurev.ph.25.030163.003125. [DOI] [PubMed] [Google Scholar]
  30. ZERAHN K. Oxygen consumption and active sodium transport in the isolated and short-circuited frog skin. Acta Physiol Scand. 1956 May 31;36(4):300–318. doi: 10.1111/j.1748-1716.1956.tb01327.x. [DOI] [PubMed] [Google Scholar]

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