Abstract
The addition of ammonium ions to the external medium results in an inhibition of the sodium influx and net uptake in Carassius auratus, while intraperitoneal injection of ammonium produces the opposite effect. The simultaneous chloride balance is not significantly affected by these treatments. The addition of bicarbonate ions to the external medium results in a reduction of the influx and net flux of chloride, while injection of bicarbonate produces the opposite effect. The simultaneous sodium balance is not significantly altered. The effects of the external additions are reversible after elimination of the excess ammonium or bicarbonate ions by rinsing. Inhibition of carbonic anhydrase in the gill by injection of acetazoleamide produces a simultaneous inhibition of both sodium and chloride exchanges. These results confirm the hypothesis of an exchange of sodium for ammonium, and of bicarbonate for chloride across the gill. A tentative schematic representation of the ionic absorption mechanisms in the branchial cell of the fresh-water teleosts is given. Similarities with other biological membranes and especially with the renal tubule are pointed out.
Full Text
The Full Text of this article is available as a PDF (1.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CLAPP J. R., WATSON J. F., BERLINER R. W. EFFECT OF CARBONIC ANHYDRASE INHIBITION ON PROXIMAL TUBULAR BICARBONATE REABSORPTION. Am J Physiol. 1963 Oct;205:693–696. doi: 10.1152/ajplegacy.1963.205.4.693. [DOI] [PubMed] [Google Scholar]
- GOLDSTEIN L., FORSTER R. P. Source of ammonia excreted by the gills of the marine teleost, Myoxocephalus scorpius. Am J Physiol. 1961 May;200:1116–1118. doi: 10.1152/ajplegacy.1961.200.5.1116. [DOI] [PubMed] [Google Scholar]
- HERNANDEZ T., COULSON R. A. The effect of carbonic anhydrase inhibition on the composition of urine and plasma of the alligator. Science. 1954 Feb 26;119(3087):291–292. doi: 10.1126/science.119.3087.291. [DOI] [PubMed] [Google Scholar]
- HODLER J., HEINEMANN H. O., FISHMAN A. P., SMITH H. W. Urine pH and carbonic anhydrase activity in the marine dogfish. Am J Physiol. 1955 Oct;183(1):155–162. doi: 10.1152/ajplegacy.1955.183.1.155. [DOI] [PubMed] [Google Scholar]
- HOGBEN C. A. Active transport of chloride by isolated frog gastric epithelium; origin of the gastric mucosal potential. Am J Physiol. 1955 Mar;180(3):641–649. [PubMed] [Google Scholar]
- MAETZ J. L'anhydrase carbonique dans seux téléostéens voisins; action des sulfamides sur la chlorémie du serran. C R Seances Soc Biol Fil. 1953 Feb;147(3-4):291–295. [PubMed] [Google Scholar]
- MAETZ J. Les échanges de sodium chez le poisson Carassius auratus L.; action d'un inhibiteur de l'anhydrase carbonique. J Physiol (Paris) 1956;48(6):1085–1099. [PubMed] [Google Scholar]
- MOREL F., BOUDIAK C. [Research on the mechanism of transport of chloride and potassium ions through the epithelium of the distal tubules of the kidney]. Helv Physiol Pharmacol Acta. 1962;20:173–192. [PubMed] [Google Scholar]
- PEQUIN L., SERFATY A. L'EXCRETION AMMONIACALE CHEZ UN TELEOSTEEN DULCICOLE: CYPRINUS CARPIO L. Comp Biochem Physiol. 1963 Dec;10:315–324. doi: 10.1016/0010-406x(63)90230-5. [DOI] [PubMed] [Google Scholar]
- RECTOR F. C., Jr, CLAPP J. R. Evidence for active chloride reabsorption in the distal renal tubule of the rat. J Clin Invest. 1962 Jan;41:101–107. doi: 10.1172/JCI104451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RECTOR F. C., Jr, SELDIN D. W., ROBERTS A. D., Jr, COPENHAVER J. H. Relation of ammonia excretion to urine pH. Am J Physiol. 1954 Nov;179(2):353–358. doi: 10.1152/ajplegacy.1954.179.2.353. [DOI] [PubMed] [Google Scholar]