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. 1964 Sep 1;48(1):163–179. doi: 10.1085/jgp.48.1.163

The Effects of Various Ions on Resting and Spike Potentials of Barnacle Muscle Fibers

Susumu Hagiwara 1,2, Shiko Chichibu 1,2, Ken-ichi Naka 1,2
PMCID: PMC2195399  PMID: 14212147

Abstract

Effects of monovalent cations and some anions on the electrical properties of the barnacle muscle fiber membrane were studied when the intra- or extracellular concentrations of those ions were altered by longitudinal intra-cellular injection. The resting potential of the normal fiber decreases linearly with increase of logarithm of [K+]out and the decrement for a tenfold increase in [K+]out is 58 mv when the product, [K+]out ·[Cl-]out, is kept constant. It also decreases with decreasing [K+]in but is always less than expected theoretically. The deviation becomes larger as [K+]in increases and the resting potential finally starts to decrease with increasing [K+]in for [K+]in > 250 mM. When the internal K+ concentration is decreased the overshoot of the spike potential increases and the time course of the spike potential becomes more prolonged. In substituting for the internal K+, Na+ and sucrose affect the resting and spike potentials similarly. Some organic cations (guanidine, choline, tris, and TMA) behave like sucrose while some other organic cations (TEA, TPA, and TBA) have a specific effect and prolong the spike potential if they are applied intracellularly or extracellularly. In all cases the active membrane potential increases linearly with the logarithm of [Ca++]out/[K+]in and the increment is about 29 mv for tenfold increase in this ratio. The fiber membrane is permeable to Cl- and other smaller anions (Br- and I-) but not to acetate- and larger anions (citrate-, sulfate-, and methanesulfonate-).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER P. F., HODGKIN A. L., SHAW T. I. Replacement of the axoplasm of giant nerve fibres with artificial solutions. J Physiol. 1962 Nov;164:330–354. doi: 10.1113/jphysiol.1962.sp007025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BAKER P. F., HODGKIN A. L., SHAW T. I. The effects of changes in internal ionic concentrations on the electrical properties of perfused giant axons. J Physiol. 1962 Nov;164:355–374. doi: 10.1113/jphysiol.1962.sp007026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FATT P., GINSBORG B. L. The ionic requirements for the production of action potentials in crustacean muscle fibres. J Physiol. 1958 Aug 6;142(3):516–543. doi: 10.1113/jphysiol.1958.sp006034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FATT P., KATZ B. The electrical properties of crustacean muscle fibres. J Physiol. 1953 Apr 28;120(1-2):171–204. doi: 10.1113/jphysiol.1953.sp004884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HAGIWARA S., NAKA K. I., CHICHIBU S. MEMBRANE PROPERTIES OF BARNACLE MUSCLE FIBER. Science. 1964 Mar 27;143(3613):1446–1448. doi: 10.1126/science.143.3613.1446. [DOI] [PubMed] [Google Scholar]
  6. HAGIWARA S., NAKA K. I. THE INITIATION OF SPIKE POTENTIAL IN BARNACLE MUSCLE FIBERS UNDER LOW INTRACELLULAR CA++. J Gen Physiol. 1964 Sep;48:141–162. doi: 10.1085/jgp.48.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOYLE G., SMYTH T., Jr NEUROMUSCULAR PHYSIOLOGY OF GIANT MUSCLE FIBERS OF A BARNACLE, BALANUS NUBILUS DARWIN. Comp Biochem Physiol. 1963 Dec;10:291–314. doi: 10.1016/0010-406x(63)90229-9. [DOI] [PubMed] [Google Scholar]
  8. KEYNES R. D., LEWIS P. R. The sodium and potassium content of cephalopod nerve fibers. J Physiol. 1951 Jun;114(1-2):151–182. doi: 10.1113/jphysiol.1951.sp004609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. NARAHASHI T. DEPENDENCE OF RESTING AND ACTION POTENTIALS ON INTERNAL POTASSIUM IN PERFUSED SQUID GIANT AXONS. J Physiol. 1963 Nov;169:91–115. doi: 10.1113/jphysiol.1963.sp007243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. OIKAWA T., SPYROPOULOS C. S., TASAKI I., TEORELL T. Methods for perfusing the giant axon of Loligo pealii. Acta Physiol Scand. 1961 Jun;52:195–196. doi: 10.1111/j.1748-1716.1961.tb02218.x. [DOI] [PubMed] [Google Scholar]
  11. TASAKI I., TAKENAKA T. RESTING AND ACTION POTENTIAL OF SQUID GIANT AXONS INTRACELLULARLY PERFUSED WITH SODIUM-RICH SOLUTIONS. Proc Natl Acad Sci U S A. 1963 Oct;50:619–626. doi: 10.1073/pnas.50.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. TASAKII, SHIMAMURA M. Further observations on resting and action potential of intracellularly perfused squid axon. Proc Natl Acad Sci U S A. 1962 Sep 15;48:1571–1577. doi: 10.1073/pnas.48.9.1571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TASAKII, WATANABE A., TAKENAKA T. Resting and action potential of intracellularly perfused squid giant axon. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1177–1184. doi: 10.1073/pnas.48.7.1177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WERMAN R., GRUNDFEST H. Graded and all-or-none electrogenesis in arthropod muscle. II. The effects of alkali-earth and onium ions on lobster muscle fibers. J Gen Physiol. 1961 May;44:997–1027. doi: 10.1085/jgp.44.5.997. [DOI] [PMC free article] [PubMed] [Google Scholar]

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