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. 1965 Mar 1;48(4):543–557. doi: 10.1085/jgp.48.4.543

Intracellular Electrical Potentials in Frog Skin

Marcelino Cereijido 1, Peter F Curran 1
PMCID: PMC2195432  PMID: 14324974

Abstract

The influence of changes in ionic composition of the bathing solutions on intracellular electrical potentials in frog skin has been examined. When the skin bathed in SO4 Ringer's solution is penetrated with a microelectrode two approximately equal potential jumps were frequently observed and most experiments were carried out with the electrode located between these steps. Substitution of Cl for SO4 in the bathing solutions caused a decrease in PD across both the "outer" and "inner" barriers. When the skin was short-circuited an average intracellular potential of -18 mv was found with both Cl and SO4 Ringer's. With the skin in SO4 Ringer's, decrease in Na concentration of the outside solution caused a decrease in PD between the microelectrode and the outside solution which was approximately the same as the decrease in total skin PD. With SO4 Ringer's, an increase in K concentration in the inside solution caused a marked decrease in total skin PD. However, only 50 per cent of this change occurred at the inner barrier, between the microelectrode and the inside solution. The remainder of the change occurred at the outer barrier. This observation does not appear to be consistent with the model of the skin proposed by Koefoed-Johnson and Ussing (Acta Physiol. Scand., 1958, 42, 298).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRICKER N. S., BIBER T., USSING H. H. Exposure of the isolated from skin to high potassium concentrations at the internal surface. I. Bioelectric phenomena and sodium transport. J Clin Invest. 1963 Jan;42:88–99. doi: 10.1172/JCI104699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CEREIJIDO M., HERRERA F. C., FLANIGAN W. J., CURRAN P. F. THE INFLUENCE OF NA CONCENTRATION ON NA TRANSPORT ACROSS FROG SKIN. J Gen Physiol. 1964 May;47:879–893. doi: 10.1085/jgp.47.5.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ENGBAEK L., HOSHIKO T. Electrical potential gradients through frog skin. Acta Physiol Scand. 1957 Jul 1;39(4):348–355. doi: 10.1111/j.1748-1716.1957.tb01433.x. [DOI] [PubMed] [Google Scholar]
  4. FRAZIER H. S., LEAF A. The electrical characteristics of active sodium transport in the toad bladder. J Gen Physiol. 1963 Jan;46:491–503. doi: 10.1085/jgp.46.3.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HANSEN H. H., ZERAHN K. CONCENTRATION OF LITHIUM, SODIUM AND POTASSIUM IN EPITHELIAL CELLS OF THE ISOLATED FROG SKIN DURING ACTIVE TRANSPORT OF LITHIUM. Acta Physiol Scand. 1964 Jan-Feb;60:189–196. doi: 10.1111/j.1748-1716.1964.tb02882.x. [DOI] [PubMed] [Google Scholar]
  6. HUF E. G., WILLS J. P., ARRIGHI M. F. Electrolyte distribution and active salt uptake in frog skin. J Gen Physiol. 1955 Jul 20;38(6):867–888. doi: 10.1085/jgp.38.6.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JOHNSEN V. K., LEVI H., USSING H. H. The mode of passage of chloride ions through the isolated frog skin. Acta Physiol Scand. 1952 Jun 6;25(2-3):150–163. doi: 10.1111/j.1748-1716.1952.tb00866.x. [DOI] [PubMed] [Google Scholar]
  8. KOEFOED-JOHNSEN V., USSING H. H. The nature of the frog skin potential. Acta Physiol Scand. 1958 Jun 2;42(3-4):298–308. doi: 10.1111/j.1748-1716.1958.tb01563.x. [DOI] [PubMed] [Google Scholar]
  9. MACROBBIE E. A., USSING H. H. Osmotic behaviour of the epithelial cells of frog skin. Acta Physiol Scand. 1961 Nov-Dec;53:348–365. doi: 10.1111/j.1748-1716.1961.tb02293.x. [DOI] [PubMed] [Google Scholar]
  10. USSING H. H., WINDHAGER E. E. NATURE OF SHUNT PATH AND ACTIVE SODIUM TRANSPORT PATH THROUGH FROG SKIN EPITHELIUM. Acta Physiol Scand. 1964 Aug;61:484–504. [PubMed] [Google Scholar]
  11. WHITTEMBURY G. ELECTRICAL POTENTIAL PROFILE OF THE TOAD SKIN EPITHELIUM. J Gen Physiol. 1964 Mar;47:795–808. doi: 10.1085/jgp.47.4.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ZADUNAISKY J. A., CANDIA O. A., CHIARANDINI D. J. THE ORIGIN OF THE SHORT-CIRCUIT CURRENT IN THE ISOLATED SKIN OF THE SOUTH AMERICAN FROG LEPTODACTYLUS OCELLATUS. J Gen Physiol. 1963 Nov;47:393–402. doi: 10.1085/jgp.47.2.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ZERAHN K. Oxygen consumption and active sodium transport in the isolated and short-circuited frog skin. Acta Physiol Scand. 1956 May 31;36(4):300–318. doi: 10.1111/j.1748-1716.1956.tb01327.x. [DOI] [PubMed] [Google Scholar]

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