Abstract
Green photosynthetic bacteria exhibit variations in the intensity of their fluorescence during illumination. The initial intensity of fluorescence, measured at the onset of illumination, has a spectrum in which the major pigment Chlorobium chlorophyll predominates. The minor pigment bacteriochlorophyll predominates in the spectrum of the time-varying part of the fluorescence. The spectrum of delayed light emission is identical to that of the time-varying fluorescence. The variations in fluorescence also resemble the delayed light in their kinetics and in their dependence on exciting light intensity. Similar results are obtained for the kinetics of prompt and delayed light emission in the algae Chlorella and Anacystis. These findings raise the possibility that the variations in fluorescence actually represent a fast component of delayed light emission, of intensity comparable to the intensity of fluorescence. In Anacystis there is an outburst of light emission that develops after the exciting light has been turned off, reaching a maximum intensity after 1 to 3 seconds. This emitted light has the spectrum of chlorophyll fluorescence. It appears to be a novel example of bioluminescence with singlet excited chlorophyll as the emitter.
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Selected References
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- ARNOLD W., DAVIDSON J. B. The identity of the fluorescent and delayed light emission spectra in Chlorella. J Gen Physiol. 1954 May 20;37(5):677–684. doi: 10.1085/jgp.37.5.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bertsch W. F. TWO PHOTOREACTIONS IN PHOTOSYNTHESIS: EVIDENCE FROM THE DELAYED LIGHT EMISSION OF CHLORELLA. Proc Natl Acad Sci U S A. 1962 Dec;48(12):2000–2004. doi: 10.1073/pnas.48.12.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOEDHEER J. C. A cooperation of two pigment systems and respiration in photosynthetic luminescence. Biochim Biophys Acta. 1963 Jan 15;66:61–71. doi: 10.1016/0006-3002(63)91167-3. [DOI] [PubMed] [Google Scholar]
- GOEDHEER J. C., VEGT G. R. Chemiluminescence of chlorophyll a and bacteriochlorophyll in relation to redox reactions. Nature. 1962 Mar 3;193:875–876. doi: 10.1038/193875a0. [DOI] [PubMed] [Google Scholar]
- OLSON J. M., ROMANO C. A. A new chlorophyll from green bacteria. Biochim Biophys Acta. 1962 Jun 4;59:726–728. doi: 10.1016/0006-3002(62)90659-5. [DOI] [PubMed] [Google Scholar]
- STREHLER B. L., ARNOLD W. Light production by green plants. J Gen Physiol. 1951 Jul;34(6):809–820. doi: 10.1085/jgp.34.6.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SYBESMA C., OLSON J. M. Transfer of chlorophyll excitation energy in green photosynthetic bacteria. Proc Natl Acad Sci U S A. 1963 Feb 15;49:248–253. doi: 10.1073/pnas.49.2.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SYBESMA C., VREDENBERG W. J. EVIDENCE FOR A REACTION CENTER P 840 IN THE GREEN PHOTOSYNTHETIC BACTERIUM CHLOROPSEUDOMONAS ETHYLICUM. Biochim Biophys Acta. 1963 Nov 29;75:439–441. doi: 10.1016/0006-3002(63)90632-2. [DOI] [PubMed] [Google Scholar]
- SYBESMA C., VREDENBERG W. J. KINETICS OF LIGHT-INDUCED CYTOCHROME OXIDATION AND P840 BLEACHING IN GREEN PHOTOSYNTHETIC BACTERIA UNDER VARIOUS CONDITIONS. Biochim Biophys Acta. 1964 Jul 29;88:205–207. doi: 10.1016/0926-6577(64)90167-6. [DOI] [PubMed] [Google Scholar]
- VREDENBERG W. J., DUYSENS L. N. Transfer of energy from bacteriochlorophyll to a reaction centre during bacterial photosynthesis. Nature. 1963 Jan 26;197:355–357. doi: 10.1038/197355a0. [DOI] [PubMed] [Google Scholar]
- WARBURG O., BURK D. The maximum efficiency of photosynthesis. Arch Biochem. 1950 Feb;25(2):410–443. [PubMed] [Google Scholar]