Abstract
The relationship between retinula and eccentric cells in the lateral eye of Limulus polyphemus was studied using a double electrode technique which permitted simultaneous recording of light-initiated responses in two sense cells and the labeling of the cells for subsequent histological examination and identification. The following results were obtained: (a) light-initiated slow responses with and without superimposed spike potentials were recorded from retinula cells and from eccentric cells (only one eccentric cell yielded responses without superimposed spike potentials); (b) spike potentials recorded in different cells within the same ommatidium were always synchronous; (c) a complete absence of spike potentials was observed in two experiments in which no eccentric cells could be found in the ommatidia containing the labeled retinula cells; (d) the greatest differences in the characteristics of responses recorded simultaneously occurred in those recorded from retinula-eccentric combinations. The results indicate that there is only one source of spike potential activity within an ommatidium (presumably the eccentric cell) and that the light-initiated response of retinula cells may be independent of the eccentric cell response. The suggestion is advanced that the response of the retinula cell may "trigger" the eccentric cell response.
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Selected References
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- FUORTES M. G. Electric activity of cells in the eye of Limulus. Am J Ophthalmol. 1958 Nov;46(5 Pt 2):210–223. doi: 10.1016/0002-9394(58)90800-6. [DOI] [PubMed] [Google Scholar]
- HARTLINE H. K., WAGNER H. G., MACNICHOL E. F., Jr The peripheral origin of nervous activity in the visual system. Cold Spring Harb Symp Quant Biol. 1952;17:125–141. doi: 10.1101/sqb.1952.017.01.013. [DOI] [PubMed] [Google Scholar]
- RAHMAN A. N., LUTTRELL C. N. Albumen embedding method for frozen sectioning of fresh tissues for histological, fluorescent antibody, and histochemical studies. Bull Johns Hopkins Hosp. 1962 Feb;110:66–72. [PubMed] [Google Scholar]
- SMITH T. G., BAUMANN F., FUORTES M. G. ELECTRICAL CONNECTIONS BETWEEN VISUAL CELLS IN THE OMMATIDIUM OF LIMULUS. Science. 1965 Mar 19;147(3664):1446–1448. doi: 10.1126/science.147.3664.1446. [DOI] [PubMed] [Google Scholar]
- TOMITA T. Peripheral mechanism of nervous activity in lateral eye of horseshoe crab. J Neurophysiol. 1957 May;20(3):245–254. doi: 10.1152/jn.1957.20.3.245. [DOI] [PubMed] [Google Scholar]
- TOMITA T. The nature of action potentials in the lateral eye of the horseshoe crab as revealed by simultaneous intra- and extracellular recording. Jpn J Physiol. 1956 Dec 31;6(4):327–340. doi: 10.2170/jjphysiol.6.327. [DOI] [PubMed] [Google Scholar]