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. 1965 Sep 1;49(1):131–149.

Calcium Binding by Particle-Free Supernatants of Homogenates of Skeletal Muscle

F Norman Briggs 1, Martin Fleishman 1
PMCID: PMC2195472  PMID: 5862499

Abstract

A high molecular weight fraction of a soluble Marsh muscle-relaxing preparation has been shown to contain a calcium-complexing substance. By examining the nature of the competition between this fraction and chelex-100 for calcium at various total calcium concentrations it has been possible to calculate the concentration and calcium stability constant of this calcium-complexing substance. Taking into account dilutions which occur during the preparation of fractions containing this substance its concentration may be estimated at about 2·10-4 in muscle and its calcium stability constant was found to be about 1.5·105 M -1. Preliminary evidence suggests that the calcium-binding substance is a protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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