Skip to main content
PMC home page
The Journal of General Physiology logoLink to The Journal of General Physiology
. 1965 Nov 1;49(2):309–320. doi: 10.1085/jgp.49.2.309

Interaction between the Effects of Inside and Outside Na and K on Bullfrog Skin Potential

Daniel E Leb 1, Charles Edwards 1, Barry D Lindley 1, T Hoshiko 1; with the technical assistance of James A. Dugan1
PMCID: PMC2195478  PMID: 5880985

Abstract

The composition of the solution bathing one border of the isolated frog skin affects the response of the potential across the skin to changes in the composition of the solution bathing the opposite border. Increasing the K concentration of the inside (corium) bathing solution decreased the sensitivity of the potential to a change in outside Na concentration. Decreasing the outside Na concentration decreased the sensitivity of the potential to a change in inside K concentration. Increasing the total ionic strength of the outside bathing solution or of both bathing solutions decreased the sensitivity of the potential to a change in outside Na concentration.

Full Text

The Full Text of this article is available as a PDF (658.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN B., ZERAHN K. METHOD FOR NON-DESTRUCTIVE DETERMINATION OF THE SODIUM TRANSPORT POOL IN FROG SKIN WITH RADIOSODIUM. Acta Physiol Scand. 1963 Dec;59:319–329. doi: 10.1111/j.1748-1716.1963.tb02747.x. [DOI] [PubMed] [Google Scholar]
  2. BRICKER N. S., BIBER T., USSING H. H. Exposure of the isolated from skin to high potassium concentrations at the internal surface. I. Bioelectric phenomena and sodium transport. J Clin Invest. 1963 Jan;42:88–99. doi: 10.1172/JCI104699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CURRAN P. F., HERRERA F. C., FLANIGAN W. J. The effect of Ca and antidiuretic hormone on Na transport across frog skin. II. Sites and mechanisms of action. J Gen Physiol. 1963 May;46:1011–1027. doi: 10.1085/jgp.46.5.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FARQUHAR M. G., PALADE G. E. FUNCTIONAL ORGANIZATION OF AMPHIBIAN SKIN. Proc Natl Acad Sci U S A. 1964 Apr;51:569–577. doi: 10.1073/pnas.51.4.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRAZIER H. S., LEAF A. The electrical characteristics of active sodium transport in the toad bladder. J Gen Physiol. 1963 Jan;46:491–503. doi: 10.1085/jgp.46.3.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HANSEN H. H., ZERAHN K. CONCENTRATION OF LITHIUM, SODIUM AND POTASSIUM IN EPITHELIAL CELLS OF THE ISOLATED FROG SKIN DURING ACTIVE TRANSPORT OF LITHIUM. Acta Physiol Scand. 1964 Jan-Feb;60:189–196. doi: 10.1111/j.1748-1716.1964.tb02882.x. [DOI] [PubMed] [Google Scholar]
  7. HOSHIKO T., LINDLEY B. D., EDWARDS C. DIFFUSION DELAY IN FROG SKIN CONNECTIVE TISSUE: A SOURCE OF ERROR IN TRACER INVESTIGATIONS. Nature. 1964 Feb 29;201:932–933. doi: 10.1038/201932a0. [DOI] [PubMed] [Google Scholar]
  8. HOSHIKO T., USSING H. H. The kinetics of Na24 flux across amphibian skin and bladder. Acta Physiol Scand. 1960 May 25;49:74–81. doi: 10.1111/j.1748-1716.1960.tb01931.x. [DOI] [PubMed] [Google Scholar]
  9. HUF E. G., WILLS J. P., ARRIGHI M. F. Electrolyte distribution and active salt uptake in frog skin. J Gen Physiol. 1955 Jul 20;38(6):867–888. doi: 10.1085/jgp.38.6.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KOEFOED-JOHNSEN V., USSING H. H. The nature of the frog skin potential. Acta Physiol Scand. 1958 Jun 2;42(3-4):298–308. doi: 10.1111/j.1748-1716.1958.tb01563.x. [DOI] [PubMed] [Google Scholar]
  11. LINDLEY B. D., HOSHIKO T., LEB D. E. EFFECTS OF D2O AND OSMOTIC GRADIENTS ON POTENTIAL AND RESISTANCE OF THE ISOLATED FROG SKIN. J Gen Physiol. 1964 Mar;47:773–793. doi: 10.1085/jgp.47.4.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LINDLEY B. D., HOSHIKO T. THE EFFECTS OF ALKALI METAL CATIONS AND COMMON ANIONS ON THE FROG SKIN POTENTIAL. J Gen Physiol. 1964 Mar;47:749–771. doi: 10.1085/jgp.47.4.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. USSING H. H., ZERAHN K. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta Physiol Scand. 1951 Aug 25;23(2-3):110–127. doi: 10.1111/j.1748-1716.1951.tb00800.x. [DOI] [PubMed] [Google Scholar]
  14. WHITTEMBURY G. ELECTRICAL POTENTIAL PROFILE OF THE TOAD SKIN EPITHELIUM. J Gen Physiol. 1964 Mar;47:795–808. doi: 10.1085/jgp.47.4.795. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of General Physiology are provided here courtesy of The Rockefeller University Press

RESOURCES