Abstract
L cells exposed to cytochalasin-B (cyto-B) show the normal development of deep cleavage furrows in both bipolar and multipolar cell divisions Due to the drug-induced inhibition of cellular motility, the resulting daughter cells do not move away from each other but reunite to form multinucleate cells. In mitotic cells from cultures exposed to cyto-B for long periods of time, vigorous blebbing and contraction of the cell surface is seen The evidence from time-lapse studies presented suggests that cyto-B-induced multinucleate cells are formed, not by the failure of the cleavage furrow, but by the drug-induced changes in surface activity and motility of cells after division.
Full Text
The Full Text of this article is available as a PDF (860.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Carter S. B. Effects of cytochalasins on mammalian cells. Nature. 1967 Jan 21;213(5073):261–264. doi: 10.1038/213261a0. [DOI] [PubMed] [Google Scholar]
- Estensen R. D., Rosenberg M., Sheridan J. D., Wessells N. K., Spooner B. S., Ash J. F., Ludueña M. A., Wrenn J. T. Cytochalasin B: microfilaments and "contractile" processes. Science. 1971 Jul 23;173(3994):356–359. doi: 10.1126/science.173.3994.356. [DOI] [PubMed] [Google Scholar]
- Goldman R. D. The effects of cytochalasin B on the microfilaments of baby hamster kidney (BHK-21) cells. J Cell Biol. 1972 Feb;52(2):246–254. doi: 10.1083/jcb.52.2.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hammer M. G., Sheridan J. D., Estensen R. D. Cytochalasin B II: selective inhibition of cytokinesis in Xenopus laevis eggs. Proc Soc Exp Biol Med. 1971 Apr;136(4):1158–1162. doi: 10.3181/00379727-136-35450. [DOI] [PubMed] [Google Scholar]
- Sanger J. W., Holtzer H. Cytochalasin B: effects on cell morphology, cell adhesion, and mucopolysaccharide synthesis (cultured cells-contractile microfilaments-glycoproteins-embryonic cells-sorting-out). Proc Natl Acad Sci U S A. 1972 Jan;69(1):253–257. doi: 10.1073/pnas.69.1.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spooner B. S., Wessells N. K. Effects of cytochalasin B upon microfilaments involved in morphogenesis of salivary epithelium. Proc Natl Acad Sci U S A. 1970 Jun;66(2):360–361. doi: 10.1073/pnas.66.2.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spooner B. S., Yamada K. M., Wessells N. K. Microfilaments and cell locomotion. J Cell Biol. 1971 Jun;49(3):595–613. doi: 10.1083/jcb.49.3.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wessells N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamada K. Microfilaments in cellular and developmental processes. Science. 1971 Jan 15;171(3967):135–143. doi: 10.1126/science.171.3967.135. [DOI] [PubMed] [Google Scholar]
- Wrenn J. T., Wessells N. K. Cytochalasin B: effects upon microfilaments involved in morphogenesis of estrogen-induced glands of oviduct. Proc Natl Acad Sci U S A. 1970 Jul;66(3):904–908. doi: 10.1073/pnas.66.3.904. [DOI] [PMC free article] [PubMed] [Google Scholar]