Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Apr;150(1):113–121. doi: 10.1128/jb.150.1.113-121.1982

Recombination between two IS/s flanking the r-determinant of R100-1: involvement of dor and recA gene functions in Salmonella typhimurium.

H Watanabe, K Mise, H Hashimoto
PMCID: PMC220089  PMID: 6277859

Abstract

Drug resistance genes of the r-determinant component of a composite R plasmid R100-1 were frequently lost in Salmonella typhimurium. Various deletion mutants were analyzed by restriction endonuclease cleavage, Southern blotting, and hybridization techniques. The loss of the r-determinant was found to be the result of a reciprocal recombination between the two IS/s flanking the r-determinant. This recombination depended upon both dor and recA gene functions.

Full text

PDF
113

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alton N. K., Vapnek D. Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature. 1979 Dec 20;282(5741):864–869. doi: 10.1038/282864a0. [DOI] [PubMed] [Google Scholar]
  2. Bochner B. R., Huang H. C., Schieven G. L., Ames B. N. Positive selection for loss of tetracycline resistance. J Bacteriol. 1980 Aug;143(2):926–933. doi: 10.1128/jb.143.2.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brachet P., Eisen H., Rambach A. Mutations of coliphage lambda affecting the expression of replicative functions O and P. Mol Gen Genet. 1970;108(3):266–276. doi: 10.1007/BF00283357. [DOI] [PubMed] [Google Scholar]
  4. Chandler M., Allet B., Gallay E., Boy de La Tour E., Caro L. Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1. Mol Gen Genet. 1977 Jun 24;153(3):289–295. doi: 10.1007/BF00431594. [DOI] [PubMed] [Google Scholar]
  5. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hopkins J. D., Clements M. B., Liang T. Y., Isberg R. R., Syvanen M. Recombination genes on the Escherichia coli sex factor specific for transposable elements. Proc Natl Acad Sci U S A. 1980 May;77(5):2814–2818. doi: 10.1073/pnas.77.5.2814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hu S., Otsubo E., Davidson N., Saedler H. Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids. J Bacteriol. 1975 May;122(2):764–775. doi: 10.1128/jb.122.2.764-775.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Iida S., Arber W. On the role of IS1 in the formation of hybrids between the bacteriophage P1 and the R plasmid NR1. Mol Gen Genet. 1980 Jan;177(2):261–270. doi: 10.1007/BF00267437. [DOI] [PubMed] [Google Scholar]
  10. Kopecko D. J., Brevet J., Cohen S. N. Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids. J Mol Biol. 1976 Dec;108(2):333–360. doi: 10.1016/s0022-2836(76)80124-6. [DOI] [PubMed] [Google Scholar]
  11. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  12. Landy A., Ross W. Viral integration and excision: structure of the lambda att sites. Science. 1977 Sep 16;197(4309):1147–1160. doi: 10.1126/science.331474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miki T., Easton A. M., Rownd R. H. Mapping of the resistance genes of the R plasmid NR1. Mol Gen Genet. 1978 Jan 17;158(3):217–224. doi: 10.1007/BF00267192. [DOI] [PubMed] [Google Scholar]
  14. Miller H. I., Friedman D. I. An E. coli gene product required for lambda site-specific recombination. Cell. 1980 Jul;20(3):711–719. doi: 10.1016/0092-8674(80)90317-7. [DOI] [PubMed] [Google Scholar]
  15. Nash H. A. Integration and excision of bacteriophage lambda. Curr Top Microbiol Immunol. 1977;78:171–199. doi: 10.1007/978-3-642-66800-5_6. [DOI] [PubMed] [Google Scholar]
  16. Ohtsubo H., Ohtsubo E. Nucleotide sequence of an insertion element, IS1. Proc Natl Acad Sci U S A. 1978 Feb;75(2):615–619. doi: 10.1073/pnas.75.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ptashne K., Cohen S. N. Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J Bacteriol. 1975 May;122(2):776–781. doi: 10.1128/jb.122.2.776-781.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  19. Ross D. G., Grisafi P., Kleckner N., Botstein D. The ends of Tn10 are not IS3. J Bacteriol. 1979 Sep;139(3):1097–1101. doi: 10.1128/jb.139.3.1097-1101.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanderson K. E., Hartman P. E. Linkage map of Salmonella typhimurium, edition V. Microbiol Rev. 1978 Jun;42(2):471–519. doi: 10.1128/mr.42.2.471-519.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sheehy R. J., Perry A., Allison D. P., Curtiss R., 3rd Molecular nature of R-factor deoxyribonucleic acid isolated from Salmonella typhimurium minicells. J Bacteriol. 1973 Jun;114(3):1328–1335. doi: 10.1128/jb.114.3.1328-1335.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Silver L., Chandler M., Lane H. E., Caro L. Production of extrachromosomal r-determinant circles from integrated R100.1: involvement of the E. coli recombination system. Mol Gen Genet. 1980;179(3):565–571. doi: 10.1007/BF00271746. [DOI] [PubMed] [Google Scholar]
  23. Tanak N., Cramer J. H., Rownd R. H. EcoRI restriction endonuclease map of the composite R plasmid NR1. J Bacteriol. 1976 Jul;127(1):619–636. doi: 10.1128/jb.127.1.619-636.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  25. Vogelstein B., Gillespie D. Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A. 1979 Feb;76(2):615–619. doi: 10.1073/pnas.76.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Watanabe H., Hashimoto H., Mitsuhashi S. Salmonella typhimurium LT2 mutation affecting the deletion of resistance determinants on R plasmids. J Bacteriol. 1980 Apr;142(1):145–152. doi: 10.1128/jb.142.1.145-152.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Watanabe T., Ogata Y. Genetic stability of various resistance factors in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1970 May;102(2):363–368. doi: 10.1128/jb.102.2.363-368.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES