Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Apr;150(1):156–160. doi: 10.1128/jb.150.1.156-160.1982

Another gene affecting sexual expression of Escherichia coli.

T J Lerner, N D Zinder
PMCID: PMC220094  PMID: 6120928

Abstract

We have examined the relationship between two chromosomal mutations of Escherichia coli K-12, fexA (0 min) and fexB (85 min), in regulating expression of the F sex factor. Together, fexA and fexB exert a pleiotropic effect on the expression of the F tra genes. F pilus synthesis, conjugal donor activity, and surface exclusion activity are all inhibited in the fexA fexB mutant. Either fex mutation alone is cryptic.

Full text

PDF
156

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Kennedy N., Skurray R. Cell--cell interactions in conjugating Escherichia coli: role of traT protein in surface exclusion. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5104–5108. doi: 10.1073/pnas.74.11.5104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beutin L., Manning P. A., Achtman M., Willetts N. sfrA and sfrB products of Escherichia coli K-12 are transcriptional control factors. J Bacteriol. 1981 Feb;145(2):840–844. doi: 10.1128/jb.145.2.840-844.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buxton R. S., Hammer-Jespersen K., Hansen T. D. Insertion of bacteriophage lambda into the deo operon of Escherichia coli K-12 and isolation of plaque-forming lambdadeo+ transducing bacteriophages. J Bacteriol. 1978 Nov;136(2):668–681. doi: 10.1128/jb.136.2.668-681.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chakrabarti S., Gorini L. Growth of bacteriophages MS2 and T7 on streptomycin-resistant mutants of Escherichia coli. J Bacteriol. 1975 Feb;121(2):670–674. doi: 10.1128/jb.121.2.670-674.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Finnegan D., Willetts N. The nature of the transfer inhibitor of several F-like plasmids. Mol Gen Genet. 1972;119(1):57–66. doi: 10.1007/BF00270444. [DOI] [PubMed] [Google Scholar]
  7. Helmuth R., Achtman M. Operon structure of DNA transfer cistrons on the F sex factor. Nature. 1975 Oct 23;257(5528):652–656. doi: 10.1038/257652a0. [DOI] [PubMed] [Google Scholar]
  8. Holland I. B., Threlfall E. J., Holland E. M., Darby V., Samson A. C. Mutants of Escherichia coli with altered surface properties which are refractory to colicin E2, sensitive to ultraviolet light and which can also show recombination deficiency, abortive growth of bacteriophage lambda and filament formation. J Gen Microbiol. 1970 Aug;62(3):371–382. doi: 10.1099/00221287-62-3-371. [DOI] [PubMed] [Google Scholar]
  9. Iyer V. N., Iyer R. V., Palchoudhury S. R., Becker S., Stevenson I. An aspect of the physiology of strains carrying a dnaB mutation. Impairment in F piliation and its phenotypic reversal. Mol Gen Genet. 1974;133(2):111–122. doi: 10.1007/BF00264832. [DOI] [PubMed] [Google Scholar]
  10. Jamieson A. F., Bergquist P. L. Genetic mapping of chromosomal mutations affecting the replication of the F-factor of Escherichia coli. Mol Gen Genet. 1976 Oct 18;148(2):221–223. doi: 10.1007/BF00268388. [DOI] [PubMed] [Google Scholar]
  11. Kennedy N., Beutin L., Achtman M., Skurray R., Rahmsdorf U., Herrlich P. Conjugation proteins encoded by the F sex factor. Nature. 1977 Dec 15;270(5638):580–585. doi: 10.1038/270580a0. [DOI] [PubMed] [Google Scholar]
  12. LOEB T., ZINDER N. D. A bacteriophage containing RNA. Proc Natl Acad Sci U S A. 1961 Mar 15;47:282–289. doi: 10.1073/pnas.47.3.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lerner T. J., Zinder N. D. Chromosomal regulation of sexual expression in Escherichia coli. J Bacteriol. 1979 Feb;137(2):1063–1065. doi: 10.1128/jb.137.2.1063-1065.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  16. McEwen J., Silverman P. Chromosomal mutations of Escherichia coli that alter expression of conjugative plasmid functions. Proc Natl Acad Sci U S A. 1980 Jan;77(1):513–517. doi: 10.1073/pnas.77.1.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McEwen J., Silverman P. Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: identification of genes cpxA and cpxB. J Bacteriol. 1980 Oct;144(1):60–67. doi: 10.1128/jb.144.1.60-67.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Novotny C. P., Taylor P. F., Lavin K. Effects of growth inhibitors and ultraviolet irradiation on F pili. J Bacteriol. 1972 Dec;112(3):1083–1089. doi: 10.1128/jb.112.3.1083-1089.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roeder W., Somerville R. L. Cloning the trpR gene. Mol Gen Genet. 1979 Nov;176(3):361–368. doi: 10.1007/BF00333098. [DOI] [PubMed] [Google Scholar]
  20. Romero E., Riva S., Berti M., Fietta A. M., Silvestri L. G. Pleiotropic effects of a rifampicin-resistant mutation in E. coli. Nat New Biol. 1973 Dec 19;246(155):225–228. doi: 10.1038/newbio246225a0. [DOI] [PubMed] [Google Scholar]
  21. Willetts N. The transcriptional control of fertility in F-like plasmids. J Mol Biol. 1977 May 5;112(1):141–148. doi: 10.1016/s0022-2836(77)80161-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES