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. 1982 Apr;150(1):214–220. doi: 10.1128/jb.150.1.214-220.1982

Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli.

Y M Chen, K Backman, B Magasanik
PMCID: PMC220101  PMID: 6120930

Abstract

DNA was prepared from a strain of Escherichia coli bearing a mutation which confers the GlnC phenotype (inability to reduce the expression of glnA and other nitrogen-regulated operons in response to ammonia in the growth medium). A fragment of this DNA carrying glnA, the structural gene for glutamine synthetase, was cloned on plasmid pBR322. By using recombination in vitro, we mapped the GlnC mutation to a region between glnA and glnG. This region defines a gene, glnL, which codes for a trans-acting product; the GlnC mutant produces an altered product. The glnL product plays a key role in the communication of information concerning the quality and abundance of the nitrogen source in the growth medium to a destination responsible for the regulation of glnA and other genes for enzymes responsible for nitrogen utilization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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