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. 1982 Apr;150(1):298–302. doi: 10.1128/jb.150.1.298-302.1982

Transfer of the gonococcal penicillinase plasmid: mobilization in Escherichia coli by IncP plasmids and isolation as a DNA-protein relaxation complex.

D G Guiney Jr, J I Ito Jr
PMCID: PMC220113  PMID: 6801022

Abstract

A 4.4-megadalton penicillinase plasmid, pWD2, from Neisseria gonorrhoeae was transformed into Escherichia coli. pWD2 was efficiently mobilized by IncP plasmids in E. coli but not by Flac, R1drd-19, or R64drd-11. pWD2 could be isolated as a DNA-protein relaxation complex with properties similar to the well characterized ColE1 complex. The host range of pWD2 was shown to include gonococci, Enterobacteriaceae, and Hemophilus influenzae, but not Acinetobacter calcoaceticus or Pseudomonas aeruginosa. These findings suggest that P-group plasmids could have played a role in the dissemination of the TEM beta-lactamase to pathogenic gram-negative bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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