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. 1968 Mar 1;51(3):427–442. doi: 10.1085/jgp.51.3.427

Effects of Potentiators of Muscular Contraction on Binding of Cations by Sarcoplasmic Reticulum

Arselio P Carvalho 1
PMCID: PMC2201136  PMID: 4231148

Abstract

Anionic (NO3 -, Br-, I-, and SCN-) and cationic (Zn++ and Cd++) potentiators of the twitch output of skeletal muscle depress the active binding of Ca by sarcoplasmic reticulum isolated from rabbit skeletal muscle. Zinc and Cd exchange for Ca and Mg at the binding sites of the reticular membranes, whereas the anions effectively induce a replacement by Mg of Ca bound actively in the presence of ATP. In the absence of ATP, the passive binding of both Ca and Mg is increased by the anions tested. Furthermore, the anions increase the total capacity of the membrane fragments for passive cation binding. The Ca-stimulated ATPase activity of the membranes is inhibited by Zn and Cd, but not by the anions. Shifts in cations bound to muscle membrane systems caused by agents that increase the force of contraction developed during the twitch are considered to be the primary event modifying excitation-contraction coupling, and thus leading to potentiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CARVALHO A. P., SANUI H., PACE N. CALCIUM AND MAGNESIUM BINDING PROPERTIES OF CELL MEMBRANE MATERIALS. J Cell Physiol. 1963 Dec;62:311–317. doi: 10.1002/jcp.1030620311. [DOI] [PubMed] [Google Scholar]
  2. Carvalho A. P. Binding of cations by microsomes from rabbit skeletal muscle. J Cell Physiol. 1966 Feb;67(1):73–83. doi: 10.1002/jcp.1040670109. [DOI] [PubMed] [Google Scholar]
  3. Carvalho A. P., Leo B. Effects of ATP on the interaction of Ca++, Mg++, and K+ with fragmented sarcoplasmic reticulum isolated from rabbit skeletal muscle. J Gen Physiol. 1967 May;50(5):1327–1352. doi: 10.1085/jgp.50.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Engel A. G., Tice L. W. Cytochemistry of phosphatases of the sarcoplasmic reticulum. I. Biochemical studies. J Cell Biol. 1966 Dec;31(3):473–487. doi: 10.1083/jcb.31.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GENT W. L., TROUNCE J. R., WALSER M. THE BINDING OF CALCIUM ION BY THE HUMAN ERYTHROCYTE MEMBRANE. Arch Biochem Biophys. 1964 Jun;105:582–589. doi: 10.1016/0003-9861(64)90054-2. [DOI] [PubMed] [Google Scholar]
  6. HODGKIN A. L., HOROWICZ P. The effect of nitrate and other anions on the mechanical response of single muscle fibres. J Physiol. 1960 Sep;153:404–412. doi: 10.1113/jphysiol.1960.sp006542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KOKETSU K., KITAMURA R., TANAKA R. BINDING OF CALCIUM IONS TO CELL MEMBRANE ISOLATED FROM BULLFROG SKELETAL MUSCLE. Am J Physiol. 1964 Aug;207:509–512. doi: 10.1152/ajplegacy.1964.207.2.509. [DOI] [PubMed] [Google Scholar]
  8. LEITCH G. J., TOBIAS J. M. PHOSPHOLIPID-CHOLESTEROL MEMBRANE MODEL: EFFECTS OF CALCIUM, POTASSIUM OR PROTAMINE ON MEMBRANE HYDRATION, WATER PERMEABILITY AND ELECTRICAL RESISTANCE. J Cell Physiol. 1964 Apr;63:225–232. doi: 10.1002/jcp.1030630213. [DOI] [PubMed] [Google Scholar]
  9. MARTONOSI A. ROLE OF PHOSPHOLIPIDS IN ATPASE ACTIVITY AND CA TRANSPORT OF FRAGMENTED SARCOPLASMIC RETICULUM. Fed Proc. 1964 Sep-Oct;23:913–921. [PubMed] [Google Scholar]
  10. MIKULECKY D. C., TOBIAS J. M. PHOSPHOLIPID-CHOLESTEROL MEMBRANE MODEL. I. CORRELATION OF RESISTANCE WITH ION CONTENT. II. CATION EXCHANGE PROPERTIES. 3. EFFECT OF CA ON SALT PERMEABILITY. IV. CA-K UPTAKE BY SONICALLY FRAGMENTED ERYTHROCYTE GHOSTS. J Cell Physiol. 1964 Oct;64:151–163. doi: 10.1002/jcp.1030640202. [DOI] [PubMed] [Google Scholar]
  11. SANDOW A. POTENTIATION OF MUSCULAR CONTRACTION. Arch Phys Med Rehabil. 1964 Feb;45:62–81. [PubMed] [Google Scholar]
  12. SANDOW A., PREISER H. MUSCULAR CONTRACTION AS REGULATED BY THE ACTION POTENTIAL. Science. 1964 Dec 11;146(3650):1470–1472. doi: 10.1126/science.146.3650.1470. [DOI] [PubMed] [Google Scholar]
  13. SANUI H., PACE N. SODIUM, POTASSIUM AND HYDROGEN ION BINDING CHARACTERISTICS OF DOG ERYTHROCYTE GHOSTS. J Cell Physiol. 1963 Aug;62:95–103. doi: 10.1002/jcp.1030620111. [DOI] [PubMed] [Google Scholar]
  14. SHANES A. M. Electrochemical aspects of physiological and pharmacological action in excitable cells. II. The action potential and excitation. Pharmacol Rev. 1958 Jun;10(2):165–273. [PubMed] [Google Scholar]
  15. Sandow A., Isaacson A. Topochemical factors in potentiation of contraction by heavy metal cations. J Gen Physiol. 1966 May;49(5):937–961. doi: 10.1085/jgp.49.5.937. [DOI] [PMC free article] [PubMed] [Google Scholar]

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