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. 1968 Apr 1;51(4):552–578. doi: 10.1085/jgp.51.4.552

The Roles of Sodium and Potassium Ions in the Generation of the Electro-Olfactogram

Sadayuki F Takagi 1, Gordon A Wyse 1, Harunobu Kitamura 1, Katsuhiro Ito 1
PMCID: PMC2201159  PMID: 5651772

Abstract

In order to clarify whether or not the electronegative olfactory mucosal potentials (EOG) are generator potentials, the effects of changed ionic enviroment were studied. The EOG decreased in amplitude and in some cases nearly or completely disappeared, when Na+ in the bathing Ringer solution was replaced by sucrose, Li+, choline+, tetraethylammonium+ (TEA), or hydrazine. In the K+-free Ringer solution, the negative EOG's initially increased and then decreased in amplitude. In Ringer's solution with increased K+, the negative EOG's increased in amplitude. When K+ was increased in exchange for Na+ in Ringer's solution, the negative EOG's decreased, disappeared, and then reversed their polarity (Fig. 6). Next, when the K+ was replaced by equimolar sucrose, Li+, choline+, TEA+, hydrazine, or Na+, the reversed potentials recovered completely only in Na+-Ringer's solution, but never in the other solutions. Thus, the essential role of Na+ and K+ in the negative EOG's was demonstrated. Ba++ was found to depress selectively the electropositive EOG, but it hardly decreased and never increased the negative EOG. Hence, it is concluded that Ba++ interferes only with Cl- influx, and that the negative EOG's are elicited by an increase in permeability of the olfactory receptive membrane to Na+ and K+, but not to Cl-. From the ionic mechanism it is inferred that the negative EOG's are in most cases composites of generator and positive potentials.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AI N., TAKAGI S. F. THE EFFECT OF ETHER AND CHLOROFORM ON THE OLFACTORY EPITHELIUM. Jpn J Physiol. 1963 Oct 15;13:454–465. doi: 10.2170/jjphysiol.13.454. [DOI] [PubMed] [Google Scholar]
  2. DIAMOND J., GRAY J. A., INMAN D. R. The relation between receptor potentials and the concentration of sodium ions. J Physiol. 1958 Jul 14;142(2):382–394. doi: 10.1113/jphysiol.1958.sp006024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FRANKENHAEUSER B., HODGKIN A. L. The action of calcium on the electrical properties of squid axons. J Physiol. 1957 Jul 11;137(2):218–244. doi: 10.1113/jphysiol.1957.sp005808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FURUKAWA T., HANAWA I. Effects of some common cations on electroretinogram of the toad. Jpn J Physiol. 1955 Dec 15;5(4):289–300. doi: 10.2170/jjphysiol.5.289. [DOI] [PubMed] [Google Scholar]
  5. GESTELAND R. C. INITIAL EVENTS OF THE ELECTRO-OLFACTOGRAM. Ann N Y Acad Sci. 1964 Jul 30;116:440–447. doi: 10.1111/j.1749-6632.1964.tb45073.x. [DOI] [PubMed] [Google Scholar]
  6. Gesteland R. C., Lettvin J. Y., Pitts W. H. Chemical transmission in the nose of the frog. J Physiol. 1965 Dec;181(3):525–559. doi: 10.1113/jphysiol.1965.sp007781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HAGIWARA S., NAKA K. I. THE INITIATION OF SPIKE POTENTIAL IN BARNACLE MUSCLE FIBERS UNDER LOW INTRACELLULAR CA++. J Gen Physiol. 1964 Sep;48:141–162. doi: 10.1085/jgp.48.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HAMASAKI D. I. The effect of sodium ion concentration on the electroretinogram of the isolated retina of the frog. J Physiol. 1963 Jun;167:156–168. doi: 10.1113/jphysiol.1963.sp007138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HIGASHINO S., TAKAGI S. F. THE EFFECT OF ELECTROTONUS ON THE OLFACTORY EPITHELIUM. J Gen Physiol. 1964 Nov;48:323–335. doi: 10.1085/jgp.48.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HIGASHINO S., TAKAGI S. F., YAJIMA M. The olfactory stimulating effectiveness of homologous series of substances studied in the frog. Jpn J Physiol. 1961 Oct 15;11:530–544. doi: 10.2170/jjphysiol.11.530. [DOI] [PubMed] [Google Scholar]
  11. HUXLEY A. F., STAMPFLI R. Effect of potassium and sodium on resting and action potentials of single myelinated nerve fibers. J Physiol. 1951 Feb;112(3-4):496–508. doi: 10.1113/jphysiol.1951.sp004546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hagiwara S., Nakajima S. Effects of the intracellular Ca ion concentration upon the excitability of the muscle fiber membrane of a barnacle. J Gen Physiol. 1966 Mar;49(4):807–818. doi: 10.1085/jgp.49.4.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KOKETSU K., CERF J. A., NISHI S. Further observations on electrical activity of frog spinal ganglion cells in sodium-free solutions. J Neurophysiol. 1959 Nov;22:693–703. doi: 10.1152/jn.1959.22.6.693. [DOI] [PubMed] [Google Scholar]
  14. Moulton D. G., Beidler L. M. Structure and function in the peripheral olfactory system. Physiol Rev. 1967 Jan;47(1):1–52. doi: 10.1152/physrev.1967.47.1.1. [DOI] [PubMed] [Google Scholar]
  15. OTTOSON D. THE EFFECT OF SODIUM DEFICIENCY ON THE RESPONSE OF THE ISOLATED MUSCLE SPINDLE. J Physiol. 1964 May;171:109–118. doi: 10.1113/jphysiol.1964.sp007365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SHIBUYA T. The electrical responses of the olfactory epithelium of some fishes. Jpn J Physiol. 1960 Jun 29;10:317–326. doi: 10.2170/jjphysiol.10.317. [DOI] [PubMed] [Google Scholar]
  17. TAKAGI S. F., SHIBUYA T., HIGASHINO S., ARAI T. The stimulative and anaesthetic actions of ether on the olfactory epithelium of the frog and the toad. Jpn J Physiol. 1960 Dec 15;10:571–584. doi: 10.2170/jjphysiol.10.571. [DOI] [PubMed] [Google Scholar]
  18. TAKAGI S. F., SHIBUYA T. The electrical activity of the olfactory epithelium studied with micro- and macro-electrodes. Jpn J Physiol. 1960 Aug 15;10:385–395. doi: 10.2170/jjphysiol.10.385. [DOI] [PubMed] [Google Scholar]
  19. TAKAGI S. F., YAJIMA T. ELECTRICAL ACTIVITY AND HISTOLOGICAL CHANGE IN THE DEGENERATING OLFACTORY EPITHELIUM. J Gen Physiol. 1965 Mar;48:559–569. doi: 10.1085/jgp.48.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. TAKAGI S. F., YAJIMA T. ELECTRICAL RESPONSES TO ODOURS OF DEGENERATING OLFACOTRY EPITHELIUM. Nature. 1964 Jun 20;202:1220–1220. doi: 10.1038/2021220a0. [DOI] [PubMed] [Google Scholar]
  21. Takagi S. F., Wyse G. A., Yajima T. Anion permeability of the olfactory receptive membrane. J Gen Physiol. 1966 Nov;50(2):473–489. doi: 10.1085/jgp.50.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tucker D., Shibuya T. A physiologic and pharmacologic study of olfactory receptors. Cold Spring Harb Symp Quant Biol. 1965;30:207–215. doi: 10.1101/sqb.1965.030.01.023. [DOI] [PubMed] [Google Scholar]

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