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. 1982 Apr;150(1):395–397. doi: 10.1128/jb.150.1.395-397.1982

Phenotypic expression of mutations in a wide-host-range R plasmid in Escherichia coli and Rhizobium meliloti.

P J Hooykaas, H den Dulk-Ras, R A Schilperoort
PMCID: PMC220127  PMID: 7037750

Abstract

Eight different derivatives of R plasmid RP1 with thermosensitive mutations affecting maintenance in Escherichia coli and Pseudomonas aeruginosa were introduce into Rhizobium meliloti. None of the plasmids showed a thermosensitive character in R. meliloti. On the other hand, a certain deletion mutation in RP1 was found to cause plasmid instability in rhizobia and agrobacteria, but not in E. coli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beringer J. E. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol. 1974 Sep;84(1):188–198. doi: 10.1099/00221287-84-1-188. [DOI] [PubMed] [Google Scholar]
  2. Danilevich V. N., Stepanshin Iu G., Volozhantsev N. V., Volkovoi K. I. Vydelenie i kharakteristika deletsionnykh mutantov temperaturochuvstvitel'noi plazmidy pEG1. Genetika. 1980;16(11):1958–1966. [PubMed] [Google Scholar]
  3. Danilevich V. N., Stepanshin Y. G., Volozhanstev N. V., Golub E. I. Transposon-mediated insertion of R factor into bacterial chromosome. Mol Gen Genet. 1978 May 31;161(3):337–339. doi: 10.1007/BF00331010. [DOI] [PubMed] [Google Scholar]
  4. Datta N., Hedges R. W. Host ranges of R factors. J Gen Microbiol. 1972 May;70(3):453–460. doi: 10.1099/00221287-70-3-453. [DOI] [PubMed] [Google Scholar]
  5. Harayama S., Tsuda M., Iino T. High frequency mobilization of the chromosome of Escherichia coli by a mutant of plasmid RP4 temperature-sensitive for maintenance. Mol Gen Genet. 1980;180(1):47–56. doi: 10.1007/BF00267351. [DOI] [PubMed] [Google Scholar]
  6. Holloway B. W. Plasmids that mobilize bacterial chromosome. Plasmid. 1979 Jan;2(1):1–19. doi: 10.1016/0147-619x(79)90002-7. [DOI] [PubMed] [Google Scholar]
  7. Hooykaas P. J., den Dulk-Ras H., Schilperoort R. A. Molecular mechanism of Ti plasmid mobilization by R plasmids: isolation of Ti plasmids with transposon-insertions in Agrobacterium tumefaciens. Plasmid. 1980 Jul;4(1):64–75. doi: 10.1016/0147-619x(80)90083-9. [DOI] [PubMed] [Google Scholar]
  8. Ilyina T. S., Nechaeva E. V., Romanova YuM, Smirnov G. B. Isolation and mapping of Escherichia coli K12 mutants defective in Tn9 transposition. Mol Gen Genet. 1981;181(3):384–389. doi: 10.1007/BF00425616. [DOI] [PubMed] [Google Scholar]
  9. Robinson M. K., Bennett P. M., Falkow S., Dodd H. M. Isolation of a temperature-sensitive derivative of RP1. Plasmid. 1980 May;3(3):343–347. doi: 10.1016/0147-619x(80)90047-5. [DOI] [PubMed] [Google Scholar]
  10. Windass J. D., Worsey M. J., Pioli E. M., Pioli D., Barth P. T., Atherton K. T., Dart E. C., Byrom D., Powell K., Senior P. J. Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus. Nature. 1980 Oct 2;287(5781):396–401. doi: 10.1038/287396a0. [DOI] [PubMed] [Google Scholar]

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