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. 1982 Apr;150(1):410–413. doi: 10.1128/jb.150.1.410-413.1982

Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans.

L A Sherman, P van de Putte
PMCID: PMC220131  PMID: 6277870

Abstract

A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicated in both organisms, had unique sites for two restriction endonucleases, conferred ampicillin resistance on both organisms, and could be used as a cloning vector in A. nidulans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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