Abstract
We demonstrated the genetic transformation of Rhizobium leguminosarum by R68.45 plasmid DNA by freezing and thawing cell suspensions in the presence of R68.45 plasmid DNA and 20 mM MgCl2. Clones resistant to kanamycin and tetracycline were recovered at a frequency of 10(-8) per recipient cell. No colonies that were doubly drug resistant were recovered in parallel control experiments.
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- Adachi T., Iyer V. N. A procedure for the isolation and purification of plasmid DNA from Rhizobium meliloti. Anal Biochem. 1980 Jan 15;101(2):271–274. doi: 10.1016/0003-2697(80)90185-2. [DOI] [PubMed] [Google Scholar]
- Balassa G. GENETIC TRANSFORMATION OF RHIZOBIUM: A REVIEW OF THE WORK OF R. BALASSA. Bacteriol Rev. 1963 Jun;27(2):228–241. doi: 10.1128/br.27.2.228-241.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beringer J. E. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol. 1974 Sep;84(1):188–198. doi: 10.1099/00221287-84-1-188. [DOI] [PubMed] [Google Scholar]
- Ditta G., Stanfield S., Corbin D., Helinski D. R. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347–7351. doi: 10.1073/pnas.77.12.7347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drozańska D., Lorkiewicz Z. Genetic transformation in Rhizobium trifolii. Acta Microbiol Pol. 1978;27(2):81–88. [PubMed] [Google Scholar]
- Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
- Holsters M., de Waele D., Depicker A., Messens E., van Montagu M., Schell J. Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet. 1978 Jul 11;163(2):181–187. doi: 10.1007/BF00267408. [DOI] [PubMed] [Google Scholar]
- Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
- Jacob A. E., Cresswell J. M., Hedges R. W., Coetzee J. N., Beringer J. E. Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4. Mol Gen Genet. 1976 Sep 23;147(3):315–323. doi: 10.1007/BF00582883. [DOI] [PubMed] [Google Scholar]
- Johnston A. W., Bibb M. J., Beringer J. E. Tryptophan genes in Rhizobium--their organization and their transfer to other bacterial genera. Mol Gen Genet. 1978 Oct 24;165(3):323–330. doi: 10.1007/BF00332533. [DOI] [PubMed] [Google Scholar]
- Neve R. L., West R. W., Rodriguez R. L. Eukaryotic DNA fragments which act as promoters for a plasmid gene. Nature. 1979 Jan 25;277(5694):324–325. doi: 10.1038/277324a0. [DOI] [PubMed] [Google Scholar]
- O'Gara F., Dunican L. K. Transformation and physical properties of R-factor RP4 transferred from Escherichia coli to Rhizobium trifolii. J Bacteriol. 1973 Dec;116(3):1177–1180. doi: 10.1128/jb.116.3.1177-1180.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts R. J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1981 Jan 10;9(1):r75–r96. doi: 10.1093/nar/9.1.213-c. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ruvkun G. B., Ausubel F. M. A general method for site-directed mutagenesis in prokaryotes. Nature. 1981 Jan 1;289(5793):85–88. doi: 10.1038/289085a0. [DOI] [PubMed] [Google Scholar]
- Selvaraj G., Iyer V. N. Genetic transformation of Rhizobium meliloti by plasmid DNA. Gene. 1981 Nov;15(2-3):279–283. doi: 10.1016/0378-1119(81)90137-2. [DOI] [PubMed] [Google Scholar]