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. 1982 Jul;151(1):29–35. doi: 10.1128/jb.151.1.29-35.1982

Nitrogen catabolite repression in a glutamate auxotroph of Saccharomyces cerevisiae.

L Kang, M L Keeler, P C Dunlop, R J Roon
PMCID: PMC220181  PMID: 6123500

Abstract

The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated without a glutamate supplement, had a diminished internal pool of alpha-ketoglutarate and a concomitant inability to equilibrate ammonium ion with alpha-amino nitrogen. In the glutamate auxotroph, asparaginase II biosynthesis exhibited a decreased sensitivity to nitrogen catabolite repression by ammonium ion but normal sensitivity to nitrogen catabolite repression by all amino acids tested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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