Abstract
A mutant strain of Escherichia coli K-12 that utilizes citrate as a sole source of carbon and energy was isolated. Citrate utilization arose as the consequence of two mutations in genes citA and citB, which are linked to the gal operon. The mutant strain expresses a semiconstitutive citrate transport system, and it utilizes both citrate and isocitrate as carbon and energy sources. It is capable of utilizing cis- and trans-aconitate, but only if it is preinduced by growth on citrate.
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Selected References
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- Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gall B. G., Hartl D. L. Regulation of newly evolved enzymes. II. The ebg repressor. Genetics. 1975 Nov;81(3):427–435. doi: 10.1093/genetics/81.3.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall B. G. Experimental evolution of a new enzymatic function. II. Evolution of multiple functions for ebg enzyme in E. coli. Genetics. 1978 Jul;89(3):453–465. doi: 10.1093/genetics/89.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall B. G., Hartl D. L. Regulation of newly evolved enzymes. I. Selection of a novel lactase regulated by lactose in Escherichia coli. Genetics. 1974 Mar;76(3):391–400. doi: 10.1093/genetics/76.3.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ishiguro N., Hirose K., Asagi M., Sato G. Incompatibility of citrate utilization plasmids isolated from Escherichia coli. J Gen Microbiol. 1981 Mar;123(1):193–196. doi: 10.1099/00221287-123-1-193. [DOI] [PubMed] [Google Scholar]
- Ishiguro N., Oka C., Hanzawa Y., Sato G. Plasmids in Escherichia coli controlling citrate-utilizing ability. Appl Environ Microbiol. 1979 Nov;38(5):956–964. doi: 10.1128/aem.38.5.956-964.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ishiguro N., Oka C., Sato G. Isolation of citrate-positive variants of Escherichia coli from domestic pigeons, pigs, cattle, and horses. Appl Environ Microbiol. 1978 Aug;36(2):217–222. doi: 10.1128/aem.36.2.217-222.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koser S. A. CORRELATION OF CITRATE UTILIZATION BY MEMBERS OF THE COLON-AEROGENES GROUP WITH OTHER DIFFERENTIAL CHARACTERISTICS AND WITH HABITAT. J Bacteriol. 1924 Jan;9(1):59–77. doi: 10.1128/jb.9.1.59-77.1924. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LARA F. J. S., STOKES J. L. Oxidation of citrate by Escherichia coli. J Bacteriol. 1952 Mar;63(3):415–420. doi: 10.1128/jb.63.3.415-420.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lo T. C., Rayman M. K., Sanwal B. D. Transport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells. J Biol Chem. 1972 Oct 10;247(19):6323–6331. [PubMed] [Google Scholar]
- Lo T. C., Sanwal B. D. Genetic analysis of mutants of Escherichia coli defective in dicarboxylate transport. Mol Gen Genet. 1975 Oct 22;140(4):303–307. doi: 10.1007/BF00267321. [DOI] [PubMed] [Google Scholar]
- Lütgens M., Gottschalk G. Why a co-substrate is required for anaerobic growth of Escherichia coli on citrate. J Gen Microbiol. 1980 Jul;119(1):63–70. doi: 10.1099/00221287-119-1-63. [DOI] [PubMed] [Google Scholar]
- Winkler H. H., Wilson T. H. The role of energy coupling in the transport of beta-galactosides by Escherichia coli. J Biol Chem. 1966 May 25;241(10):2200–2211. [PubMed] [Google Scholar]
- Wu T. T. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. doi: 10.1093/genetics/54.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]