Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Jul;151(1):495–499. doi: 10.1128/jb.151.1.495-499.1982

Identification of the Salmonella typhimurium cysB gene product by two-dimensional protein electrophoresis.

E W Baptist, S G Hallquist, N M Kredich
PMCID: PMC220270  PMID: 7045085

Abstract

Examination of two-dimensional electropherograms of proteins from wild-type Salmonella typhimurium and 16 different cysB strains permitted the identification of a single 34,500-dalton polypeptide chain with a pI of 7.6 that was the product of cysB. Exclusion chromatography indicated that the native cysB protein is a multimer of at least two and probably four or more such subunits.

Full text

PDF
495

Images in this article

496Image
on p.496
497Image
on p.497

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker M. A., Kredich N. M., Tomkins G. M. The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium. J Biol Chem. 1969 May 10;244(9):2418–2427. [PubMed] [Google Scholar]
  2. Cheney R. W., Jr, Kredich N. M. Fine-structure genetic map of the cysB locus in Salmonella typhimurium. J Bacteriol. 1975 Dec;124(3):1273–1281. doi: 10.1128/jb.124.3.1273-1281.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hulanicka M. D., Hallquist S. G., Kredich N. M., Mojica-A T. Regulation of O-acetylserine sulfhydrylase B by L-cysteine in Salmonella typhimurium. J Bacteriol. 1979 Oct;140(1):141–146. doi: 10.1128/jb.140.1.141-146.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hulanicka M. D., Kredich N. M., Treiman D. M. The structural gene for O-acetylserine sulfhydrylase A in Salmonella typhimurium. Identity with the trzA locus. J Biol Chem. 1974 Feb 10;249(3):867–872. [PubMed] [Google Scholar]
  5. Jagura-Burdzy G., Hulanicka D. Use of gene fusions to study expression of cysB, the regulatory gene of the cysteine regulon. J Bacteriol. 1981 Sep;147(3):744–751. doi: 10.1128/jb.147.3.744-751.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jagura G., Hulanicka D. Analysis of merodiploids of the cysB region in Salmonella typhimurium. Mol Gen Genet. 1978 Sep 20;165(1):31–38. doi: 10.1007/BF00270373. [DOI] [PubMed] [Google Scholar]
  7. Jones-Mortimer M. C. Positive control of sulphate reduction in Escherichia coli. The nature of the pleiotropic cysteineless mutants of E. coli K12. Biochem J. 1968 Dec;110(3):597–602. doi: 10.1042/bj1100597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones-Mortimer M. C., Wheldrake J. F., Pasternak C. A. The control of sulphate reduction in Escherichia coli by O-acetyl-L-serine. Biochem J. 1968 Mar;107(1):51–53. doi: 10.1042/bj1070051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kredich N. M., Becker M. A., Tomkins G. M. Purification and characterization of cysteine synthetase, a bifunctional protein complex, from Salmonella typhimurium. J Biol Chem. 1969 May 10;244(9):2428–2439. [PubMed] [Google Scholar]
  10. Kredich N. M. Regulation of L-cysteine biosynthesis in Salmonella typhimurium. I. Effects of growth of varying sulfur sources and O-acetyl-L-serine on gene expression. J Biol Chem. 1971 Jun 10;246(11):3474–3484. [PubMed] [Google Scholar]
  11. Kredich N. M., Tomkins G. M. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium. J Biol Chem. 1966 Nov 10;241(21):4955–4965. [PubMed] [Google Scholar]
  12. Mascarenhas D. M., Yudkin M. D. Identification of a positive regulatory protein in Escherichia coli: the product of the cysB gene. Mol Gen Genet. 1980 Feb;177(3):535–539. doi: 10.1007/BF00271494. [DOI] [PubMed] [Google Scholar]
  13. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  14. Tully M., Yudkin M. D. Fine-structure mapping and complementation analysis of the Escherichia coli cysB gene. J Bacteriol. 1977 Jul;131(1):49–56. doi: 10.1128/jb.131.1.49-56.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tully M., Yudkin M. D. The nature of the product of the cys B gene of Escherichia coli. Mol Gen Genet. 1975;136(2):181–183. doi: 10.1007/BF00272038. [DOI] [PubMed] [Google Scholar]
  16. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES