Abstract
The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahydrofolate. These results suggest that C. thermoautotrophicum synthesizes acetate from CO2 via a formate-tetrahydrofolate-corrinoid pathway.
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- Andreesen J. R., Gottschalk G., Schlegel H. G. Clostridium formicoaceticum nov. spec. isolation, description and distinction from C. aceticum and C. thermoaceticum. Arch Mikrobiol. 1970;72(2):154–174. doi: 10.1007/BF00409521. [DOI] [PubMed] [Google Scholar]
- Andreesen J. R., Ljungdahl L. G. Nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase from Clostridium thermoaceticum: purification and properties. J Bacteriol. 1974 Oct;120(1):6–14. doi: 10.1128/jb.120.1.6-14.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Andreesen J. R., Schaupp A., Neurauter C., Brown A., Ljungdahl L. G. Fermentation of glucose, fructose, and xylose by Clostridium thermoaceticum: effect of metals on growth yield, enzymes, and the synthesis of acetate from CO 2 . J Bacteriol. 1973 May;114(2):743–751. doi: 10.1128/jb.114.2.743-751.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BERNHAUER K., BECHER E., WILHARM G. Biosynthesen in der Cobalamin-Reihe. V. Uber die Umwandlung verschiedener Cobalamin-Analoga in Vitamin B12. Arch Biochem Biophys. 1959 Jul;83(1):248–258. doi: 10.1016/0003-9861(59)90030-x. [DOI] [PubMed] [Google Scholar]
- Braun K., Gottschalk G. Effect of molecular hydrogen and carbon dioxide on chemo-organotrophic growth of Acetobacterium woodii and Clostridium aceticum. Arch Microbiol. 1981 Jan;128(3):294–298. doi: 10.1007/BF00422533. [DOI] [PubMed] [Google Scholar]
- Braun M., Schoberth S., Gottschalk G. Enumeration of bacteria forming acetate from H2 and CO2 in anaerobic habitats. Arch Microbiol. 1979 Mar 12;120(3):201–204. doi: 10.1007/BF00423066. [DOI] [PubMed] [Google Scholar]
- Diekert G. B., Thauer R. K. Carbon monoxide oxidation by Clostridium thermoaceticum and Clostridium formicoaceticum. J Bacteriol. 1978 Nov;136(2):597–606. doi: 10.1128/jb.136.2.597-606.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drake H. L. Demonstration of hydrogenase in extracts of the homoacetate-fermenting bacterium Clostridium thermoaceticum. J Bacteriol. 1982 May;150(2):702–709. doi: 10.1128/jb.150.2.702-709.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drake H. L., Hu S. I., Wood H. G. Purification of carbon monoxide dehydrogenase, a nickel enzyme from Clostridium thermocaceticum. J Biol Chem. 1980 Aug 10;255(15):7174–7180. [PubMed] [Google Scholar]
- Drake H. L., Hu S. I., Wood H. G. Purification of five components from Clostridium thermoaceticum which catalyze synthesis of acetate from pyruvate and methyltetrahydrofolate. Properties of phosphotransacetylase. J Biol Chem. 1981 Nov 10;256(21):11137–11144. [PubMed] [Google Scholar]
- Elliott J. I., Brewer J. M. The inactivation of yeast enolase by 2,3-butanedione. Arch Biochem Biophys. 1978 Sep;190(1):351–357. doi: 10.1016/0003-9861(78)90285-0. [DOI] [PubMed] [Google Scholar]
- Ghambeer R. K., Wood H. G., Schulman M., Ljungdahl L. Total synthesis of acetate from CO2. 3. Inhibition by alkylhalides of the synthesis from CO2, methyltetrahydrofolate, and methyl-B12 by Clostridium thermoaceticum. Arch Biochem Biophys. 1971 Apr;143(2):471–484. doi: 10.1016/0003-9861(71)90232-3. [DOI] [PubMed] [Google Scholar]
- Irion E., Ljungdahl L. Isolation of factor 3m coenzyme and cobyric acid coenzyme plus other B12 factors from Clostridium thermoaceticum. Biochemistry. 1965 Dec;4(12):2780–2790. doi: 10.1021/bi00888a031. [DOI] [PubMed] [Google Scholar]
- Leonhardt U., Andreesen J. R. Some properties of formate dehydrogenase, accumulation and incorporation of 185W-tungsten into proteins of Clostridium formicoaceticum. Arch Microbiol. 1977 Dec 15;115(3):277–284. doi: 10.1007/BF00446453. [DOI] [PubMed] [Google Scholar]
- Ljungdahl L. G., Andreesen J. R. Formate dehydrogenase, a selenium--tungsten enzyme from Clostridium thermoaceticum. Methods Enzymol. 1978;53:360–372. doi: 10.1016/s0076-6879(78)53042-5. [DOI] [PubMed] [Google Scholar]
- Ljungdahl L. G., O'Brien W. E., Moore M. R., Liu M. T. Methylenetetrahydrofolate dehydrogenase from Clostridium formicoaceticum and methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase (combined) from Clostridium thermoaceticum. Methods Enzymol. 1980;66:599–609. doi: 10.1016/0076-6879(80)66513-6. [DOI] [PubMed] [Google Scholar]
- O'Brien W. E., Ljungdahl L. G. Fermentation of fructose and synthesis of acetate from carbon dioxide by Clostridium formicoaceticum. J Bacteriol. 1972 Feb;109(2):626–632. doi: 10.1128/jb.109.2.626-632.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RABINOWITZ J. C., PRICER W. E., Jr Formyltetrahydrofolate synthetase. I. Isolation and crystallization of the enzyme. J Biol Chem. 1962 Sep;237:2898–2902. [PubMed] [Google Scholar]
- Schulman M., Ghambeer R. K., Ljungdahl L. G., Wood H. G. Total synthesis of acetate from CO2. VII. Evidence with Clostridium thermoaceticum that the carboxyl of acetate is derived from the carboxyl of pyruvate by transcarboxylation and not by fixation of CO2. J Biol Chem. 1973 Sep 25;248(18):6255–6261. [PubMed] [Google Scholar]
- Sun A. Y., Ljungdahl L., Wood H. G. Total synthesis of acetate from CO2. II. Purification and properties of formyltetrahydrofolate synthetase from Clostridium thermoaceticum. J Bacteriol. 1969 May;98(2):842–844. doi: 10.1128/jb.98.2.842-844.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tanner R. S., Wolfe R. S., Ljungdahl L. G. Tetrahydrofolate enzyme levels in Acetobacterium woodii and their implication in the synthesis of acetate from CO2. J Bacteriol. 1978 May;134(2):668–670. doi: 10.1128/jb.134.2.668-670.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thauer R. K. CO(2)-reduction to formate by NADPH. The initial step in the total synthesis of acetate from CO(2) in Clostridium thermoaceticum. FEBS Lett. 1972 Oct 15;27(1):111–115. doi: 10.1016/0014-5793(72)80421-6. [DOI] [PubMed] [Google Scholar]
- WOOD H. G. Fermentation of 3, 4-C14-and 1-C14-labeled glucose by Clostridium thermoaceticum. J Biol Chem. 1952 Dec;199(2):579–583. [PubMed] [Google Scholar]