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. 1982 Jul;151(1):524–527. doi: 10.1128/jb.151.1.524-527.1982

Bacillus subtilis DNA gyrase: purification of subunits and reconstitution of supercoiling activity.

E Orr, W L Staudenbauer
PMCID: PMC220277  PMID: 6282812

Abstract

DNA gyrase from Bacillus subtilis 168 was purified by affinity chromatography on novobiocin-Sepharose and shown to consist of two subunits, A and B, with molecular weights of 100,000 and 85,000, respectively. The B subunits, which contains novobiocin-sensitive. ATPase activity, could complement the gyrA protein of Escherichia coli. No complementation was detected between the A subunit and the E. coli gyrB protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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