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. 1969 Feb 1;53(2):133–156. doi: 10.1085/jgp.53.2.133

The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes

Thomas E Andreoli 1, Vincent W Dennis 1, Ann M Weigl 1
PMCID: PMC2202904  PMID: 5764743

Abstract

This paper reports the effects of amphotericin B, a polyene antibiotic, on the water and nonelectrolyte permeability of optically black, thin lipid membranes formed from sheep red blood cell lipids dissolved in decane. The permeability coefficients for the diffusion of water and nonelectrolytes (PDDi) were estimated from unidirectional tracer fluxes when net water flow (Jw) was zero. Alternatively, an osmotic water permeability coefficient (Pf) was computed from Jw when the two aqueous phases contained unequal solute concentrations. In the absence of amphotericin B, when the membrane solutions contained equimolar amounts of cholesterol and phospholipid, Pf was 22.9 ± 4.6 µsec-1 and P DDHDH2O was 10.8 ± 2.4 µsec-1. Furthermore, PDDi was < 0.05 µsec-1 for urea, glycerol, ribose, arabinose, glucose, and sucrose, and σi, the reflection coefficient of each of these solutes was one. When amphotericin B (10-6 M) was present in the aqueous phases and the membrane solutions contained equimolar amounts of cholesterol and phospholipid, P DDHDH2O was 18.1 ± 2.4 µsec-1; Pf was 549 ± 143 µsec-1 when glucose, sucrose, and raffinose were the aqueous solutes. Concomitantly, PDDi varied inversely, and σi directly, with the effective hydrodynamic radii of the solutes tested. These polyene-dependent phenomena required the presence of cholesterol in the membrane solutions. These data were analyzed in terms of restricted diffusion and filtration through uniform right circular cylinders, and were compatible with the hypothesis that the interactions of amphotericin B with membrane-bound cholesterol result in the formation of pores whose equivalent radii are in the range 7 to 10.5 A.

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Selected References

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  1. Andreoli T. E., Monahan M. The interaction of polyene antibiotics with thin lipid membranes. J Gen Physiol. 1968 Aug;52(2):300–325. doi: 10.1085/jgp.52.2.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cass A., Finkelstein A. Water permeability of thin lipid membranes. J Gen Physiol. 1967 Jul;50(6):1765–1784. doi: 10.1085/jgp.50.6.1765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DURBIN R. P., FRANK H., SOLOMON A. K. Water flow through frog gastric mucosa. J Gen Physiol. 1956 Mar 20;39(4):535–551. doi: 10.1085/jgp.39.4.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DURBIN R. P. Osmotic flow of water across permeable cellulose membranes. J Gen Physiol. 1960 Nov;44:315–326. doi: 10.1085/jgp.44.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dainty J., Ginzburg B. Z. Irreversible thermodynamics and frictional models of membrane processes, with particular reference to the cell membrane. J Theor Biol. 1963 Sep;5(2):256–265. doi: 10.1016/0022-5193(63)90063-8. [DOI] [PubMed] [Google Scholar]
  6. Finkelstein A., Cass A. Effect of cholesterol on the water permeability of thin lipid membranes. Nature. 1967 Nov 18;216(5116):717–718. doi: 10.1038/216717a0. [DOI] [PubMed] [Google Scholar]
  7. GINZBURG B. Z., KATCHALSKY A. THE FRICTIONAL COEFFICIENTS OF THE FLOWS OF NON-ELECTROLYTES THROUGH ARTIFICIAL MEMBRANES. J Gen Physiol. 1963 Nov;47:403–418. doi: 10.1085/jgp.47.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HUANG C., WHEELDON L., THOMPSON T. E. THE PROPERTIES OF LIPID BILAYER MEMBRANES SEPARATING TWO AQUEOUS PHASES: FORMATION OF A MEMBRANE OF SIMPLE COMPOSITION. J Mol Biol. 1964 Jan;8:148–160. doi: 10.1016/s0022-2836(64)80155-8. [DOI] [PubMed] [Google Scholar]
  9. Hanai T., Haydon D. A., Taylor J. The influence of lipid composition and of some adsorbed proteins on the capacitance of black hydrocarbon membranes. J Theor Biol. 1965 Nov;9(3):422–432. doi: 10.1016/0022-5193(65)90041-x. [DOI] [PubMed] [Google Scholar]
  10. Hanai T., Haydon D. A. The permeability to water of bimolecular lipid membranes. J Theor Biol. 1966 Aug;11(3):370–382. doi: 10.1016/0022-5193(66)90099-3. [DOI] [PubMed] [Google Scholar]
  11. Huang C., Thompson T. E. Properties of lipid bilayer membranes separating two aqueous phases: water permeability. J Mol Biol. 1966 Feb;15(2):539–554. doi: 10.1016/s0022-2836(66)80126-2. [DOI] [PubMed] [Google Scholar]
  12. KOEFOED-JOHNSEN V., USSING H. H. The contributions of diffusion and flow to the passage of D2O through living membranes; effect of neurohypophyseal hormone on isolated anuran skin. Acta Physiol Scand. 1953 Mar 31;28(1):60–76. doi: 10.1111/j.1748-1716.1953.tb00959.x. [DOI] [PubMed] [Google Scholar]
  13. Kinsky S. C., Luse S. A., van Deenen L. L. Interaction of polyene antibiotics with natural and artificial membrane systems. Fed Proc. 1966 Sep-Oct;25(5):1503–1510. [PubMed] [Google Scholar]
  14. Longuet-Higgins H. C., Austin G. The kinetics of osmotic transport through pores of molecular dimensions. Biophys J. 1966 Mar;6(2):217–224. doi: 10.1016/S0006-3495(66)86652-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PAGANELLI C. V., SOLOMON A. K. The rate of exchange of tritiated water across the human red cell membrane. J Gen Physiol. 1957 Nov 20;41(2):259–277. doi: 10.1085/jgp.41.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PAPPENHEIMER J. R., RENKIN E. M., BORRERO L. M. Filtration, diffusion and molecular sieving through peripheral capillary membranes; a contribution to the pore theory of capillary permeability. Am J Physiol. 1951 Oct;167(1):13–46. doi: 10.1152/ajplegacy.1951.167.1.13. [DOI] [PubMed] [Google Scholar]
  17. PRESCOTT D. M., ZEUTHEN E. Comparison of water diffusion and water filtration across cell surfaces. Acta Physiol Scand. 1953 Mar 31;28(1):77–94. doi: 10.1111/j.1748-1716.1953.tb00960.x. [DOI] [PubMed] [Google Scholar]
  18. ROBBINS E., MAURO A. Experimental study of the independence of diffusion and hydrodynamic permeability coefficients in collodion membranes. J Gen Physiol. 1960 Jan;43:523–532. doi: 10.1085/jgp.43.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SCHULTZ S. G., SOLOMON A. K. Determination of the effective hydrodynamic radii of small molecules by viscometry. J Gen Physiol. 1961 Jul;44:1189–1199. doi: 10.1085/jgp.44.6.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Soll A. H. A new approach to molecular configuration applied to aqueous pore transport. J Gen Physiol. 1967 Dec;50(11):2565–2578. doi: 10.1085/jgp.50.11.2565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Solomon A. K. Characterization of biological membranes by equivalent pores. J Gen Physiol. 1968 May;51(5 Suppl):335S+–335S+. [PubMed] [Google Scholar]

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