Skip to main content
The Journal of General Physiology logoLink to The Journal of General Physiology
. 1969 Mar 1;53(3):279–297. doi: 10.1085/jgp.53.3.279

Ionic Conductances of the Surface and Transverse Tubular Membranes of Frog Sartorius Fibers

Robert S Eisenberg 1, Peter W Gage 1
PMCID: PMC2202906  PMID: 5767333

Abstract

The resting ionic conductances of frog sartorius muscle fibers have been determined in a variety of conditions in order to measure the potassium conductance of the tubular and surface membranes (g K t and g K s) and the chloride conductance of the tubular and surface membranes (g Cl t and g Cl s). In both normal fibers and fibers without tubules, measurements of input resistance and diameter were made at normal pH and at low pH when the chloride conductance was very small. These measurements permitted the separation of the ionic conductances: g Cl s = 219 µmhos/cm2; g Cl t = 0 µmhos/cm2; g K s = 28 µmhos/cm2; g K t = 55 µmhos/cm2. Possible sources of systematic error are discussed and a statistical analysis of the effects of random error is presented. The implications of the nonuniformity of membrane properties are discussed along with possible anatomical explanations.

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Freygang W. H., Jr, Rapoport S. I., Peachey L. D. Some relations between changes in the linear electrical properties of striated muscle fibers and changes in ultrastructure. J Gen Physiol. 1967 Nov;50(10):2437–2458. doi: 10.1085/jgp.50.10.2437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HILL D. K. THE SPACE ACCESSIBLE TO ALBUMIN WITHIN THE STRIATED MUSCLE FIBRE OF THE TOAD. J Physiol. 1964 Dec;175:275–294. doi: 10.1113/jphysiol.1964.sp007517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HODGKIN A. L., HOROWICZ P. The effect of sudden changes in ionic concentrations on the membrane potential of single muscle fibres. J Physiol. 1960 Sep;153:370–385. doi: 10.1113/jphysiol.1960.sp006540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HODGKIN A. L., HOROWICZ P. The influence of potassium and chloride ions on the membrane potential of single muscle fibres. J Physiol. 1959 Oct;148:127–160. doi: 10.1113/jphysiol.1959.sp006278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HUXLEY H. E. EVIDENCE FOR CONTINUITY BETWEEN THE CENTRAL ELEMENTS OF THE TRIADS AND EXTRACELLULAR SPACE IN FROG SARTORIUS MUSCLE. Nature. 1964 Jun 13;202:1067–1071. doi: 10.1038/2021067b0. [DOI] [PubMed] [Google Scholar]
  6. Sommer J. R., Johnson E. A. Cardiac muscle. A comparative study of Purkinje fibers and ventricular fibers. J Cell Biol. 1968 Mar;36(3):497–526. doi: 10.1083/jcb.36.3.497. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of General Physiology are provided here courtesy of The Rockefeller University Press

RESOURCES